ArticlePloS one2010
High-resolution genotyping via whole genome hybridizations to microarrays containing long oligonucleotide probes.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Construction of a high density SNP linkage map of kelp (Saccharina japonica) by sequencing Taq I site associated DNA and mapping of a sex determining locus.BMC genomics · 2015Article
- Distribution, functional impact, and origin mechanisms of copy number variation in the barley genome.Genome biology · 2013Article
- Extensive genomic characterization of a set of near-isogenic lines for heterotic QTL in maize (Zea mays L.).BMC genomics · 2013Article
- Vitis phylogenomics: hybridization intensities from a SNP array outperform genotype calls.PloS one · 2013Article
- Integrating cereal genomics to support innovation in the Triticeae.Functional & integrative genomics · 2012Review
- B73-Mo17 near-isogenic lines demonstrate dispersed structural variation in maize.Plant physiology · 2011Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To date, microarray-based genotyping of large, complex plant genomes has been complicated by the need to perform genome complexity reduction to obtain sufficiently strong hybridization signals. Genome complexity reduction techniques are, however, tedious and can introduce unwanted variables into genotyping assays. Here, we report a microarray-based genotyping technology for complex genomes (such as the 2.3 GB maize genome) that does not require genome complexity reduction prior to hybridization. Approximately 200,000 long oligonucleotide probes were identified as being polymorphic between the inbred parents of a mapping population and used to genotype two recombinant inbred lines. While multiple hybridization replicates provided ∼97% accuracy, even a single replicate provided ∼95% accuracy. Genotyping accuracy was further increased to >99% by utilizing information from adjacent probes. This microarray-based method provides a simple, high-density genotyping approach for large, complex genomes.
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Registered trials
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