ArticlePloS one2010
Constitutive activation of PrfA tilts the balance of Listeria monocytogenes fitness towards life within the host versus environmental survival.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 73 citations in OpenAlex.
- Understanding the role of pyruvate dehydrogenase inInfection and immunity · 2026Article
- PrfA, the master virulence regulator of Listeria monocytogenes: structural-functional insights, regulatory architecture, and antivirulence strategies.Molecular biology reports · 2026Review
- Host methylglyoxal activates theProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Growth on corn salad leaves and polystyrene as well as the temperature influence the transcriptome ofCurrent research in food science · 2026Article
- Transcriptome Sequencing Reveals Distinct Stress Response of Listeria monocytogenes 6179 During Logarithmic and Stationary Growth Phases.MicrobiologyOpen · 2025Article
- Transcriptional responses of Listeria monocytogenes to low-temperature stress reveal distinct adaptive mechanisms in the absence of BKD and PrfA activation.Microbial pathogenesis · 2025Article
- Listeria monocytogenes requires phosphotransferase systems to facilitate intracellular growth and virulence.PLoS pathogens · 2025Article
- Article
- An insight into the role of branched-chain α-keto acid dehydrogenase (BKD) complex in branched-chain fatty acid biosynthesis and virulence ofJournal of bacteriology · 2024Article
- The chaperone PrsA2 regulates the secretion, stability, and folding of listeriolysin O duringmBio · 2024Article
- Article
- Review
- Role of FruR transcriptional regulator in virulence of Listeria monocytogenes and identification of its regulon.PloS one · 2022Article
- Identification of Listeria monocytogenes Genes Contributing to Oxidative Stress Resistance under Conditions Relevant to Host Infection.Infection and immunity · 2021Article
- Differential Modulation of Listeria monocytogenes Fitness,Applied and environmental microbiology · 2020Article
- Free Fatty Acids Interfere with the DNA Binding Activity of the Virulence Regulator PrfA of Listeria monocytogenes.Journal of bacteriology · 2020Article
- Contributions of a LysR Transcriptional Regulator to Listeria monocytogenes Virulence and Identification of Its Regulons.Journal of bacteriology · 2020Article
- Article
- The σFrontiers in microbiology · 2020Review
- Characterization of the pathogenesis and immune response to Listeria monocytogenes strains isolated from a sustained national outbreak.Scientific reports · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
PrfA is a key regulator of Listeria monocytogenes pathogenesis and induces the expression of multiple virulence factors within the infected host. PrfA is post-translationally regulated such that the protein becomes activated upon bacterial entry into the cell cytosol. The signal that triggers PrfA activation remains unknown, however mutations have been identified (prfA* mutations) that lock the protein into a high activity state. In this report we examine the consequences of constitutive PrfA activation on L. monocytogenes fitness both in vitro and in vivo. Whereas prfA* mutants were hyper-virulent during animal infection, the mutants were compromised for fitness in broth culture and under conditions of stress. Broth culture prfA*-associated fitness defects were alleviated when glycerol was provided as the principal carbon source; under these conditions prfA* mutants exhibited a competitive advantage over wild type strains. Glycerol and other three carbon sugars have been reported to serve as primary carbon sources for L. monocytogenes during cytosolic growth, thus prfA* mutants are metabolically-primed for replication within eukaryotic cells. These results indicate the critical need for environment-appropriate regulation of PrfA activity to enable L. monocytogenes to optimize bacterial fitness inside and outside of host cells.
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