ArticlePloS one2010
Protein markers for insulin-producing beta cells with higher glucose sensitivity.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- Expression of Pfkfb isoenzymes during in vitro differentiation of mouse embryonic stem cells into insulin-producing cells.Turkish journal of medical sciences · 2023Article
- Multienzyme activity profiling for evaluation of cell-to-cell variability of metabolic state.FASEB bioAdvances · 2022Article
- Pancreatic β-cell heterogeneity in health and diabetes: classes, sources, and subtypes.American journal of physiology. Endocrinology and metabolism · 2021Review
- Biosynthetic Activity Differs Between Islet Cell Types and in Beta Cells Is Modulated by Glucose and Not by Secretion.Endocrinology · 2021Article
- In silico approach to predict pancreatic β-cells classically secreted proteins.Bioscience reports · 2020Article
- Gene loci associated with insulin secretion in islets from non-diabetic mice.The Journal of clinical investigation · 2019Article
- Glucose-dependent phosphorylation signaling pathways and crosstalk to mitochondrial respiration in insulin secreting cells.Cell communication and signaling : CCS · 2019Article
- Astroglial DJ-1 over-expression up-regulates proteins involved in redox regulation and is neuroprotective in vivo.Redox biology · 2018Article
- An analytical comparison of three immunoassay platforms for subpicomolar detection of protein biomarker GAD65.PloS one · 2018Article
- Heterogeneity in the Beta-Cell Population: a Guided Search Into Its Significance in Pancreas and in Implants.Current diabetes reports · 2017Review
- Development of an Enhanced Sensitivity Bead-Based Immunoassay for Real-Time In Vivo Detection of Pancreatic β-Cell Death.Endocrinology · 2015Article
- Quantitative proteomics of rat and human pancreatic beta cells.Data in brief · 2015Article
- L-cysteine reversibly inhibits glucose-induced biphasic insulin secretion and ATP production by inactivating PKM2.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells.Journal of diabetes research · 2015Article
- Effects of genetics and in utero diet on murine pancreatic development.The Journal of endocrinology · 2014Article
- The pancreatic β-cell transcriptome and integrated-omics.Current opinion in endocrinology, diabetes, and obesity · 2014Review
- Direct measurements of oscillatory glycolysis in pancreatic islet β-cells using novel fluorescence resonance energy transfer (FRET) biosensors for pyruvate kinase M2 activity.The Journal of biological chemistry · 2013Article
- Potential of protein phosphatase inhibitor 1 as biomarker of pancreatic β-cell injury in vitro and in vivo.Diabetes · 2013Article
- Minireview: progress and challenges in proteomics data management, sharing, and integration.Molecular endocrinology (Baltimore, Md.) · 2012Review
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and methodologyPancreatic beta cells show intercellular differences in their metabolic glucose sensitivity and associated activation of insulin production. To identify protein markers for these variations in functional glucose sensitivity, rat beta cell subpopulations were flow-sorted for their level of glucose-induced NAD(P)H and their proteomes were quantified by label-free data independent alternate scanning LC-MS. Beta cell-selective proteins were also identified through comparison with rat brain and liver tissue and with purified islet alpha cells, after geometrical normalization using 6 stably expressed reference proteins. PRINCIPAL
findingsAll tissues combined, 943 proteins were reliably quantified. In beta cells, 93 out of 467 quantifiable proteins were uniquely detected in this cell type; several other proteins presented a high molar abundance in beta cells. The proteome of the beta cell subpopulation with high metabolic and biosynthetic responsiveness to 7.5 mM glucose was characterized by (i) an on average 50% higher expression of protein biosynthesis regulators such as 40S and 60S ribosomal constituents, NADPH-dependent protein folding factors and translation elongation factors; (ii) 50% higher levels of enzymes involved in glycolysis and in the cytosolic arm of the malate/aspartate-NADH-shuttle. No differences were noticed in mitochondrial enzymes of the Krebs cycle, beta-oxidation or respiratory chain.
conclusionsQuantification of subtle variations in the proteome using alternate scanning LC-MS shows that beta cell metabolic glucose responsiveness is mostly associated with higher levels of glycolytic but not of mitochondrial enzymes.
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