ArticlemBio2010
Vitamin B6 is required for full motility and virulence in Helicobacter pylori.
Article in mBio, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 216 citations in OpenAlex.
- Outer Membrane Vesicles Mediate the Secretion and Nuclear Trafficking of a Bacterial Nucleomodulin.Journal of extracellular vesicles · 2026Article
- Integrative profiling ofMicrobial genomics · 2026Article
- CPP-PNA Conjugate-Mediated Inhibition ofInternational journal of molecular sciences · 2026Article
- RdxA-independent mechanism ofFrontiers in microbiology · 2025Article
- Revealing fitness and virulence determinants of hypervirulentFrontiers in cellular and infection microbiology · 2025Article
- Vitamin B6 inhibits activity ofJournal of enzyme inhibition and medicinal chemistry · 2024Article
- High salt condition alters LPS synthesis and induces the emergence of drug resistance mutations inAntimicrobial agents and chemotherapy · 2024Article
- Campylobacter jejuni and casein hydrolysate addition: Impact on poultry in vitro cecal microbiota and metabolome.PloS one · 2024Article
- Article
- Functional and structural properties of pyridoxal reductase (PdxI) from Escherichia coli: a pivotal enzyme in the vitamin B6 salvage pathway.The FEBS journal · 2023Article
- Pyridoxal 5'-phosphate synthesis and salvage in Bacteria and Archaea: predicting pathway variant distributions and holes.Microbial genomics · 2023Article
- Comparative Genomic Analysis of Fusobacterium necrophorum Provides Insights into Conserved Virulence Genes.Microbiology spectrum · 2022Article
- Actinobacillus utilizes a binding protein-dependent ABC transporter to acquire the active form of vitamin BThe Journal of biological chemistry · 2021Article
- Article
- The multifaceted role of vitamin BOpen biology · 2020Review
- Essential Metabolic Routes as a Way to ESKAPE From Antibiotic Resistance.Frontiers in public health · 2020Article
- Allosteric feedback inhibition of pyridoxine 5'-phosphate oxidase fromThe Journal of biological chemistry · 2019Article
- Review
- Vitamin Biosynthesis as an Antifungal Target.Journal of fungi (Basel, Switzerland) · 2018Review
- Growth medium-dependent antimicrobial activity of early stage MEP pathway inhibitors.PloS one · 2018Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite recent advances in our understanding of how Helicobacter pylori causes disease, the factors that allow this pathogen to persist in the stomach have not yet been fully characterized. To identify new virulence factors in H. pylori, we generated low-infectivity variants of a mouse-colonizing H. pylori strain using the classical technique of in vitro attenuation. The resulting variants and their highly infectious progenitor bacteria were then analyzed by global gene expression profiling. The gene expression levels of five open reading frames (ORFs) were significantly reduced in low-infectivity variants, with the most significant changes observed for ORFs HP1583 and HP1582. These ORFs were annotated as encoding homologs of the Escherichia coli vitamin B(6) biosynthesis enzymes PdxA and PdxJ. Functional complementation studies with E. coli confirmed H. pylori PdxA and PdxJ to be bona fide homologs of vitamin B(6) biosynthesis enzymes. Importantly, H. pylori PdxA was required for optimal growth in vitro and was shown to be essential for chronic colonization in mice. In addition to having a well-known metabolic role, vitamin B(6) is necessary for the synthesis of glycosylated flagella and for flagellum-based motility in H. pylori. Thus, for the first time, we identify vitamin B(6) biosynthesis enzymes as novel virulence factors in bacteria. Interestingly, pdxA and pdxJ orthologs are present in a number of human pathogens, but not in mammalian cells. We therefore propose that PdxA/J enzymes may represent ideal candidates for therapeutic targets against bacterial pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.