ArticleMolecular vision2010
Spatiotemporal expression pattern of ceramide kinase-like in the mouse retina.
Article in Molecular vision, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- Article
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- Article
- Hyperautofluorescent Dots are Characteristic in Ceramide Kinase Like-associated Retinal Degeneration.Scientific reports · 2019Article
- Expression in retinal neurons of fukutin and FKRP, the protein products of two dystroglycanopathy-causative genes.Molecular vision · 2018Article
- The ocular toxicity and pharmacokinetics of simvastatin following intravitreal injection in mice.International journal of ophthalmology · 2017Article
- Electrophysiological and Histological Characterization of Rod-Cone Retinal Degeneration and Microglia Activation in a Mouse Model of Mucopolysaccharidosis Type IIIB.Scientific reports · 2015Article
- Autosomal recessive cone-rod dystrophy associated with compound heterozygous mutations in the EYS gene.Documenta ophthalmologica. Advances in ophthalmology · 2014Article
- Article
- Specific sphingolipid content decrease in Cerkl knockdown mouse retinas.Experimental eye research · 2013Article
- CERKL knockdown causes retinal degeneration in zebrafish.PloS one · 2013Article
- Expression and localization of CERKL in the mammalian retina, its response to light-stress, and relationship with NeuroD1 gene.Experimental eye research · 2013Article
- Ceramide kinase-like (CERKL) interacts with neuronal calcium sensor proteins in the retina in a cation-dependent manner.Investigative ophthalmology & visual science · 2012Article
- Morphology and immunoreactivity of retrogradely double-labeled ganglion cells in the mouse retina.Investigative ophthalmology & visual science · 2011Article
- Beyond the cherry-red spot: Ocular manifestations of sphingolipid-mediated neurodegenerative and inflammatory disorders.Survey of ophthalmologyReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe CERKL gene encodes for ceramide kinase-like, a novel protein of unknown function. CERKL mutations are associated with a severe retinal phenotype. The purpose of this work was to investigate alternative splicing, and the temporal and spatial expression pattern of CERKL in the mouse retina.
methodsReverse Transcription-Polymerase Chain Reaction (RT-PCR) analysis of mouse retina RNA was used to study the expression of Cerkl at various developmental time points, and to identify its various splice-isoforms. A specific anti-CERKL antibody was developed and used for immunostaining to study the localization of the endogenous CERKL protein in retina-derived cell lines and in the mouse retina.
resultsCerkl is expressed in the mouse eye as early as embryonic day 14. A total of seven different Cerkl splice-isoforms were identified in the mouse retina. The subcellular localization of CERKL in retina-derived cell lines is variable: CERKL is diffusely distributed in the cytoplasm, and in many cells, it is highly concentrated in the perinuclear region. In most, but not all cells, CERKL is also highly concentrated in the nucleus. In the mouse retina, CERKL is located in the ganglion cell layer, in amacrine cells of the inner nuclear layer, and in photoreceptors. CERKL is highly expressed in cone photoreceptors; however, its expression level in rod photoreceptors is very low. In cultured cells, CERKL is detected in the nucleus, but in retinal cells in situ, it is mostly located in the cytoplasm.
conclusionsThe expression of Cerkl in both mature and embryonic mouse retina and the severe retinal phenotype associated with human CERKL mutations indicate that this gene plays a crucial role in retinal activity, and that it may be important for retinal development as well. The high expression level of CERKL in cones correlates with the CERKL-associated phenotype in humans. Whether nucleocytoplasmic transport of CERKL actually occurs in vivo under certain conditions and its functional significance remain to be discovered.
Indexed as
Identifiers
21151604PMC3000240W2306804992What OpenQuestion holds
Registered trials
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