ArticleInternational journal of molecular sciences2010
Thermal denaturation and aggregation of myosin subfragment 1 isoforms with different essential light chains.
Article in International journal of molecular sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed.
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- Thermal Characterisation and Isoconversional Kinetic Analysis of Osmotically Dried Pork Meat ProteinsFoods (Basel, Switzerland) · 2023Article
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- Al (III) metal augment thermal aggregation and fibrillation in protein: Role of metal toxicity in neurological diseases.Saudi journal of biological sciences · 2020Article
- Exploration of Protein Unfolding by Modelling Calorimetry Data from Reheating.Scientific reports · 2017Article
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- Heat-induced conformational changes of TET peptidase from crenarchaeon Desulfurococcus kamchatkensis.European biophysics journal : EBJ · 2015Article
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- Monomeric 14-3-3ζ has a chaperone-like activity and is stabilized by phosphorylated HspB6.Biochemistry · 2012Article
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5 authors.
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Abstract
We compared thermally induced denaturation and aggregation of two isoforms of the isolated myosin head (myosin subfragment 1, S1) containing different "essential" (or "alkali") light chains, A1 or A2. We applied differential scanning calorimetry (DSC) to investigate the domain structure of these two S1 isoforms. For this purpose, a special calorimetric approach was developed to analyze the DSC profiles of irreversibly denaturing multidomain proteins. Using this approach, we revealed two calorimetric domains in the S1 molecule, the more thermostable domain denaturing in two steps. Comparing the DSC data with temperature dependences of intrinsic fluorescence parameters and S1 ATPase inactivation, we have identified these two calorimetric domains as motor domain and regulatory domain of the myosin head, the motor domain being more thermostable. Some difference between the two S1 isoforms was only revealed by DSC in thermal denaturation of the regulatory domain. We also applied dynamic light scattering (DLS) to analyze the aggregation of S1 isoforms induced by their thermal denaturation. We have found no appreciable difference between these S1 isoforms in their aggregation properties under ionic strength conditions close to those in the muscle fiber (in the presence of 100 mM KCl). Under these conditions kinetics of this process was independent of protein concentration, and the aggregation rate was limited by irreversible denaturation of the S1 motor domain.
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