ArticlePLoS biology2010
Co-evolution of transcriptional silencing proteins and the DNA elements specifying their assembly.
Article in PLoS biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 0 citations in OpenAlex.
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- Correlation Between Improved Mating Efficiency and Weakened Scaffold-Kinase Interaction in the Mating Pheromone Response Pathway Revealed by Interspecies Complementation.Frontiers in microbiology · 2022Article
- A small targeting domain in Ty1 integrase is sufficient to direct retrotransposon integration upstream of tRNA genes.The EMBO journal · 2020Article
- Interplay of Chimeric Mating-Type Loci Impairs Fertility Rescue and Accounts for Intra-Strain Variability inFrontiers in genetics · 2019Article
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- Repair of UV-induced DNA lesions in natural Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu-Sir4 interaction.Nucleic acids research · 2017Article
- An Evolutionary Perspective on Yeast Mating-Type Switching.Genetics · 2017Review
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- Histone Deacetylases with Antagonistic Roles in Saccharomyces cerevisiae Heterochromatin Formation.Genetics · 2016Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- The Chromatin and Transcriptional Landscape of Native Saccharomyces cerevisiae Telomeres and Subtelomeric Domains.Genetics · 2015Article
- Review
- The molecular topography of silenced chromatin in Saccharomyces cerevisiae.Genes & development · 2014Article
- Global analysis of transcription factor-binding sites in yeast using ChIP-Seq.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Highly expressed loci are vulnerable to misleading ChIP localization of multiple unrelated proteins.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- High quality de novo sequencing and assembly of the Saccharomyces arboricolus genome.BMC genomics · 2013Article
- Comparative gene expression between two yeast species.BMC genomics · 2013Article
- Direct interplay among histones, histone chaperones, and a chromatin boundary protein in the control of histone gene expression.Molecular and cellular biology · 2012Article
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Authors and funding
4 authors at 3 institutions in 1 country.
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Abstract
Co-evolution of transcriptional regulatory proteins and their sites of action has been often hypothesized but rarely demonstrated. Here we provide experimental evidence of such co-evolution in yeast silent chromatin, a finding that emerged from studies of hybrids formed between two closely related Saccharomyces species. A unidirectional silencing incompatibility between S. cerevisiae and S. bayanus led to a key discovery: asymmetrical complementation of divergent orthologs of the silent chromatin component Sir4. In S. cerevisiae/S. bayanus interspecies hybrids, ChIP-Seq analysis revealed a restriction against S. cerevisiae Sir4 associating with most S. bayanus silenced regions; in contrast, S. bayanus Sir4 associated with S. cerevisiae silenced loci to an even greater degree than did S. cerevisiae's own Sir4. Functional changes in silencer sequences paralleled changes in Sir4 sequence and a reduction in Sir1 family members in S. cerevisiae. Critically, species-specific silencing of the S. bayanus HMR locus could be reconstituted in S. cerevisiae by co-transfer of the S. bayanus Sir4 and Kos3 (the ancestral relative of Sir1) proteins. As Sir1/Kos3 and Sir4 bind conserved silencer-binding proteins, but not specific DNA sequences, these rapidly evolving proteins served to interpret differences in the two species' silencers presumably involving emergent features created by the regulatory proteins that bind sequences within silencers. The results presented here, and in particular the high resolution ChIP-Seq localization of the Sir4 protein, provided unanticipated insights into the mechanism of silent chromatin assembly in yeast.
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