ArticleMolecular and cellular biology2011
Genomic mRNA profiling reveals compensatory mechanisms for the requirement of the essential splicing factor U2AF.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- A Comprehensive Analysis of the Alternative Splicing Co-Factor U2AF65B Gene Family Reveals Its Role in Stress Responses and Root Development.International journal of molecular sciences · 2025Article
- Evolution of the Early Spliceosomal Complex-From Constitutive to Regulated Splicing.International journal of molecular sciences · 2021Review
- Fission Yeast Schizosaccharomyces pombe: A Unicellular "Micromammal" Model Organism.Current protocols · 2021Article
- Early splicing functions of fission yeast Prp16 and its unexpected requirement for gene Silencing is governed by intronic features.RNA biology · 2019Article
- Long-read sequencing of nascent RNA reveals coupling among RNA processing events.Genome research · 2018Article
- The histone variant H2A.Z promotes splicing of weak introns.Genes & development · 2017Article
- The Fission Yeast Pre-mRNA-processing Factor 18 (prp18+) Has Intron-specific Splicing Functions with Links to G1-S Cell Cycle Progression.The Journal of biological chemistry · 2016Article
- RNA structure in splicing: An evolutionary perspective.RNA biology · 2016Review
- Prp4 Kinase Grants the License to Splice: Control of Weak Splice Sites during Spliceosome Activation.PLoS genetics · 2016Article
- Regulation of toll-like receptor signaling by the SF3a mRNA splicing complex.PLoS genetics · 2015Article
- Genomic functions of U2AF in constitutive and regulated splicing.RNA biology · 2015Article
- Mechanisms for U2AF to define 3' splice sites and regulate alternative splicing in the human genome.Nature structural & molecular biology · 2014Article
- Characterization and in vivo functional analysis of the Schizosaccharomyces pombe ICLN gene.Molecular and cellular biology · 2014Article
- Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.Eukaryotic cell · 2013Article
- Splicing functions and global dependency on fission yeast slu7 reveal diversity in spliceosome assembly.Molecular and cellular biology · 2013Article
- Triplex DNA-binding proteins are associated with clinical outcomes revealed by proteomic measurements in patients with colorectal cancer.Molecular cancer · 2012Article
- Evolution of a tissue-specific splicing network.Genes & development · 2011Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The large subunit of the U2 auxiliary factor (U2AF) recognizes the polypyrimidine tract (Py-tract) located adjacent to the 3' splice site to facilitate U2 snRNP recruitment. While U2AF is considered essential for pre-mRNA splicing, its requirement for splicing on a genome-wide level has not been analyzed. Using Solexa sequencing, we performed mRNA profiling for splicing in the Schizosaccharomyces pombe U2AF(59) (prp2.1) temperature-sensitive mutant. Surprisingly, our analysis revealed that introns show a range of splicing defects in the mutant strain. While U2AF(59) inactivation (nonpermissive) conditions inhibit splicing of some introns, others are spliced apparently normally. Bioinformatics analysis indicated that U2AF(59)-insensitive introns have stronger 5' splice sites and higher A/U content. Most importantly, features that contribute to U2AF(59) insensitivity of an intron unexpectedly reside in its 5'-most 30 nucleotides. These include the 5' splice site, a guanosine at position 7, and the 5' splice site-to-branch point sequence context. A differential requirement (similar to U2AF(59)) for introns may also apply to other general splicing factors (e.g., prp10). Our combined results indicate that U2AF insensitivity is a common phenomenon and that varied intron features support the existence of unrecognized aspects of spliceosome assembly.
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