ArticleThe Journal of cell biology2010
RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway.
Article in The Journal of cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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Who cites it
42 citing papers in PubMed, 75 citations in OpenAlex.
- A Syd and RUFY dynein adaptor complex mediates axonal circulation of dense core vesicles.The Journal of cell biology · 2026Article
- VPS41 recruits biosynthetic LAMP-positive vesicles through interaction with Arl8b.The Journal of cell biology · 2025Article
- Synaptic vesicle characterization of iPSC-derived dopaminergic neurons provides insight into distinct secretory vesicle pools.NPJ Parkinson's disease · 2025Article
- An optimized fluorescent reporter enables rapid and cost-effective quantification of regulated secretion from neuroendocrine cells.Frontiers in endocrinology · 2025Article
- Identification of the functional domain of the dense core vesicle biogenesis factor HID-1.PloS one · 2023Article
- Parkinson disease-associated Leucine-rich repeat kinase regulates UNC-104-dependent axonal transport of Arl8-positive vesicles iniScience · 2022Article
- SNX5 targets a monoamine transporter to the TGN for assembly into dense core vesicles by AP-3.The Journal of cell biology · 2022Article
- Neurodegenerative VPS41 variants inhibit HOPS function and mTORC1-dependent TFEB/TFE3 regulation.EMBO molecular medicine · 2021Article
- AP-3 adaptor complex-mediated vesicle trafficking.Biophysics reports · 2021Article
- Pancreatic β-Cell-Specific Deletion of VPS41 Causes Diabetes Due to Defects in Insulin Secretion.Diabetes · 2021Article
- AP-3 vesicle uncoating occurs after HOPS-dependent vacuole tethering.The EMBO journal · 2020Article
- Differential sorting behavior for soluble and transmembrane cargoes at theMolecular biology of the cell · 2020Article
- EIPR1 controls dense-core vesicle cargo retention and EARP complex localization in insulin-secreting cells.Molecular biology of the cell · 2020Article
- New factors for protein transport identified by a genome-wide CRISPRi screen in mammalian cells.The Journal of cell biology · 2019Article
- The road to lysosome-related organelles: Insights from Hermansky-Pudlak syndrome and other rare diseases.Traffic (Copenhagen, Denmark) · 2019Review
- Synaptic Vesicle Recycling Pathway Determines Neurotransmitter Content and Release Properties.Neuron · 2019Article
- Regulation of axon repulsion by MAX-1 SUMOylation and AP-3.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi.Nature communications · 2018Article
- Coupling of microtubule motors with AP-3 generated organelles in axons by NEEP21 family member calcyon.Molecular biology of the cell · 2018Article
- Neuronal functions of adaptor complexes involved in protein sorting.Current opinion in neurobiology · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
The regulated release of proteins depends on their inclusion within large dense-core vesicles (LDCVs) capable of regulated exocytosis. LDCVs form at the trans-Golgi network (TGN), but the mechanism for protein sorting to this regulated secretory pathway (RSP) and the cytosolic machinery involved in this process have remained poorly understood. Using an RNA interference screen in Drosophila melanogaster S2 cells, we now identify a small number of genes, including several subunits of the heterotetrameric adaptor protein AP-3, which are required for sorting to the RSP. In mammalian neuroendocrine cells, loss of AP-3 dysregulates exocytosis due to a primary defect in LDCV formation. Previous work implicated AP-3 in the endocytic pathway, but we find that AP-3 promotes sorting to the RSP within the biosynthetic pathway at the level of the TGN. Although vesicles with a dense core still form in the absence of AP-3, they contain substantially less synaptotagmin 1, indicating that AP-3 concentrates the proteins required for regulated exocytosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.