ArticleThe Journal of biological chemistry2011
Mechanism of down-regulation of RNA polymerase III-transcribed non-coding RNA genes in macrophages by Leishmania.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- The paradigm of intracellular parasite survival and drug resistance in leishmanial parasite through genome plasticity and epigenetics: Perception and future perspective.Frontiers in cellular and infection microbiology · 2023Review
- The pathogenicity and virulence of Leishmania - interplay of virulence factors with host defenses.Virulence · 2022Review
- Effects of functionally diverse calpain system on immune cells.Immunologic research · 2021Review
- Non-Coding RNAs in the Etiology and Control of Major and Neglected Human Tropical Diseases.Frontiers in immunology · 2021Review
- Molecular signatures of anthroponotic cutaneous leishmaniasis in the lesions of patients infected with Leishmania tropica.Scientific reports · 2020Article
- Review
- Critical role of calpain in inflammation.Biomedical reports · 2016Article
- Maf1, A New PTEN Target Linking RNA and Lipid Metabolism.Trends in endocrinology and metabolism: TEM · 2016Review
- Computing Molecular Devices in L.major through Transcriptome Analysis: Structured Simulation Approach.PloS one · 2016Article
- Involvement of RNA Polymerase III in Immune Responses.Molecular and cellular biology · 2015Article
- Regulating maf1 expression and its expanding biological functions.PLoS genetics · 2015Article
- Leishmania amazonensis exhibits phosphatidylserine-dependent procoagulant activity, a process that is counteracted by sandfly saliva.Memorias do Instituto Oswaldo Cruz · 2013Article
- Article
- Alu elements: know the SINEs.Genome biology · 2011Review
- Heat shock factor binding in Alu repeats expands its involvement in stress through an antisense mechanism.Genome biology · 2011Article
- Regulation of pol III transcription by nutrient and stress signaling pathways.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The parasitic protozoan Leishmania invades mammalian macrophages to establish infection. We reported previously that Leishmania manipulates the expression of several non-coding RNA genes (e.g. Alu RNA, B1 RNA, and signal recognition particle RNA) in macrophages to favor the establishment of their infection in the phagolysosomes of these cells (Ueda, Y., and Chaudhuri, G. (2000) J. Biol. Chem. 275, 19428-19432; Misra, S., Tripathi, M. K., and Chaudhuri, G. (2005) J. Biol. Chem. 280, 29364-29373). We report here the mechanism of this down-regulation. We found that the non-coding RNA (ncRNA) genes that are repressed by Leishmania infection in macrophages contain a "B-box" in their promoters and thus require the polymerase III transcription factor TFIIIC for their expression. We also found that Leishmania promastigotes through their surface protease (leishmanolysin or gp63) activate the thrombin receptor PAR1 in the macrophages. This activation of PAR1 raised the cytosolic concentration of Ca(2+) into the micromolar range, thereby activating the Ca(2+)-dependent protease μ-calpain. μ-Calpain then degraded TFIIIC110 to inhibit the expression of the selected ncRNA genes. Avirulent stocks of Leishmania not expressing surface gp63 failed to down-regulate ncRNAs in the exposed macrophages. Inhibition of PAR1 or calpain 1 in macrophages made them resistant to Leishmania infection. These data suggest that macrophage PAR1 and calpain 1 are potential drug targets against leishmaniasis.
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