ArticleThe Journal of biological chemistry2011
Molecular cloning and characterization of a beta-L-Arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- Bifidobacterial GH146 β-L-arabinofuranosidase for the removal of β1,3-L-arabinofuranosides on plant glycans.Applied microbiology and biotechnology · 2024Article
- Recent advances in enzymatic properties, preparation methods, and functions of glycoside hydrolase from Bifidobacterium: a review.World journal of microbiology & biotechnology · 2023Review
- Assimilation of arabinogalactan side chains with novel 3-Microbiome research reports · 2023Article
- Potential applications of recombinant bifidobacterial proteins in the food industry, biomedicine, process innovation and glycobiology.Food science and biotechnology · 2021Review
- Captive Common Marmosets (Callithrix jacchus) Are Colonized throughout Their Lives by a Community ofmBio · 2021Article
- Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes.Biochemical Society transactions · 2021Review
- Novel 3-Applied and environmental microbiology · 2021Article
- Cracking the "Sugar Code": A Snapshot ofFrontiers in plant science · 2021Review
- Plant Glycan Metabolism by Bifidobacteria.Frontiers in microbiology · 2021Review
- Crystal structure of β-L-arabinobiosidase belonging to glycoside hydrolase family 121.PloS one · 2020Article
- Extensin arabinoside chain length is modulated in elongating cotton fibre.Cell surface (Amsterdam, Netherlands) · 2019Article
- Two Novel α-l-Arabinofuranosidases fromApplied and environmental microbiology · 2019Article
- Article
- Comparative Genomics Reveals the Regulatory Complexity of Bifidobacterial Arabinose and Arabino-Oligosaccharide Utilization.Frontiers in microbiology · 2018Article
- Discovery and characterization of family 39 glycoside hydrolases from rumen anaerobic fungi with polyspecific activity on rare arabinosyl substrates.The Journal of biological chemistry · 2017Article
- Article
- Expression ofBiotechnology for biofuels · 2017Article
- Comparative Metagenomics of Cellulose- and Poplar Hydrolysate-Degrading Microcosms from Gut Microflora of the Canadian Beaver (Frontiers in microbiology · 2017Article
- Proteinaceous Molecules Mediating Bifidobacterium-Host Interactions.Frontiers in microbiology · 2016Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extensin is a glycoprotein that is rich in hydroxyprolines linked to β-L-arabinofuranosides. In this study, we cloned a hypBA2 gene that encodes a novel β-L-arabinobiosidase from Bifidobacterium longum JCM 1217. This enzyme does not have any sequence similarity with other glycoside hydrolase families but has 38-98% identity to hypothetical proteins in Bifidobacterium and Xanthomonas strains. The recombinant enzyme liberated L-arabinofuranose (Araf)-β1,2-Araf disaccharide from carrot extensin, potato lectin, and Araf-β1,2-Araf-β1,2-Araf-β-Hyp (Ara(3)-Hyp) but not Araf-α1,3-Araf-β1,2-Araf-β1,2-Araf-β-Hyp (Ara(4)-Hyp) or Araf-β1,2-Araf-β-Hyp (Ara(2)-Hyp), which indicated that it was specific for unmodified Ara(3)-Hyp substrate. The enzyme also transglycosylated 1-alkanols with retention of the anomeric configuration. This is the first report of an enzyme that hydrolyzes Hyp-linked β-L-arabinofuranosides, which defines a new family of glycoside hydrolases, glycoside hydrolase family 121.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.