ArticleMolecular & cellular proteomics : MCP2011
Revisiting rat spermatogenesis with MALDI imaging at 20-microm resolution.
Article in Molecular & cellular proteomics : MCP, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 86 citations in OpenAlex.
- MALDI Matrix: Origins, Innovations, and Frontiers.Chemical reviews · 2026Review
- A Drastic Shift in Lipid Adducts in Colon Cancer Detected by MALDI-IMS Exposes Alterations in Specific KCancers · 2021Article
- Metabolomics profiling of methamphetamine addicted human serum and three rat brain areas.RSC advances · 2019Article
- A Simple Sonication Improves Protein Signal in Matrix-Assisted Laser Desorption Ionization Imaging.Journal of the American Society for Mass Spectrometry · 2018Article
- Advances in MALDI imaging mass spectrometry of proteins in cardiac tissue, including the heart valve.Biochimica et biophysica acta. Proteins and proteomics · 2017Review
- Probing the Relationship Between Detected Ion Intensity, Laser Fluence, and Beam Profile in Thin Film and Tissue in MALDI MSI.Journal of the American Society for Mass Spectrometry · 2016Article
- Potential of MALDI imaging for the toxicological evaluation of environmental pollutants.Journal of proteomics · 2016Review
- High-resolution MALDI mass spectrometric imaging of lipids in the mammalian retina.Histochemistry and cell biology · 2015Article
- Top-down proteomics with mass spectrometry imaging: a pilot study towards discovery of biomarkers for neurodevelopmental disorders.PloS one · 2014Article
- Optimization of whole-body zebrafish sectioning methods for mass spectrometry imaging.Journal of biomolecular techniques : JBT · 2013Article
- Distribution and visualisation of chlorhexidine within the skin using ToF-SIMS: a potential platform for the design of more efficacious skin antiseptic formulations.Pharmaceutical research · 2013Article
- Current status and future perspectives of mass spectrometry imaging.International journal of molecular sciences · 2013Review
- Towards understanding region-specificity of triplet repeat diseases: coupled immunohistology and mass spectrometry imaging.Methods in molecular biology (Clifton, N.J.) · 2013Article
- MALDI-imaging segmentation is a powerful tool for spatial functional proteomic analysis of human larynx carcinoma.Journal of cancer research and clinical oncology · 2013Article
- Stretched tissue mounting for MALDI mass spectrometry imaging.Analytical chemistry · 2011Article
- Recent advances in single-cell MALDI mass spectrometry imaging and potential clinical impact.Expert review of proteomics · 2011Review
- MALDI imaging mass spectrometry for direct tissue analysis: technological advancements and recent applications.Histochemistry and cell biology · 2011Review
- Matrix sublimation/recrystallization for imaging proteins by mass spectrometry at high spatial resolution.Analytical chemistry · 2011Article
- Efficient spatial segmentation of large imaging mass spectrometry datasets with spatially aware clustering.Bioinformatics (Oxford, England) · 2011Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption/ionization (MALDI) molecular imaging technology attracts increasing attention in the field of biomarker discovery. The unambiguous correlation between histopathology and MALDI images is a key feature for success. MALDI imaging mass spectrometry (MS) at high definition thus calls for technological developments that were established by a number of small steps. These included tissue and matrix preparation steps, dedicated lasers for MALDI imaging, an increase of the robustness against cell debris and matrix sublimation, software for precision matching of molecular and microscopic images, and the analysis of MALDI imaging data using multivariate statistical methods. The goal of these developments is to approach single cell resolution with imaging MS. Currently, a performance level of 20-μm image resolution was achieved with an unmodified and commercially available instrument for proteins detected in the 2-16-kDa range. The rat testis was used as a relevant model for validating and optimizing our technological developments. Indeed, testicular anatomy is among the most complex found in mammalian bodies. In the present study, we were able to visualize, at 20-μm image resolution level, different stages of germ cell development in testicular seminiferous tubules; to provide a molecular correlate for its well established stage-specific classification; and to identify proteins of interest using a top-down approach and superimpose molecular and immunohistochemistry images.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.