ArticleNucleic acids research2011
Analysis of Escherichia coli RNase E and RNase III activity in vivo using tiling microarrays.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed.
- The Quorum Sensing Regulated sRNA Lrs1 Is Involved in the Adaptation to Low Iron in Pseudomonas aeruginosa.Environmental microbiology reports · 2025Article
- The membrane-targeting-sequence motif is required for exhibition of recessive resurrection in Escherichia coli RNase E.Nucleic acids research · 2025Article
- RNase-mediated reprogramming of Yersinia virulence.PLoS pathogens · 2024Article
- A conserved protein inhibitor brings under check the activity of RNase E in cyanobacteria.Nucleic acids research · 2024Article
- Methodologies for bacterial ribonuclease characterization using RNA-seq.FEMS microbiology reviews · 2023Review
- Fitness and Functional Landscapes of the E. coli RNase III Gene rnc.Molecular biology and evolution · 2023Article
- Characterization of RNA-based and protein-only RNases P from bacteria encoding both enzyme types.RNA (New York, N.Y.) · 2023Article
- The recognition of structured elements by a conserved groove distant from domains associated with catalysis is an essential determinant of RNase E.Nucleic acids research · 2023Article
- RNase III Participates in the Adaptation to Temperature Shock and Oxidative Stress inMicroorganisms · 2022Article
- Article
- Regulation of mRNA decay inCritical reviews in biochemistry and molecular biology · 2022Article
- CsrA regulation via binding to the base-pairing small RNA Spot 42.Molecular microbiology · 2022Article
- Inactivation of RNase P in Escherichia coli significantly changes post-transcriptional RNA metabolism.Molecular microbiology · 2022Article
- Transcriptome-wide in vivo mapping of cleavage sites for the compact cyanobacterial ribonuclease E reveals insights into its function and substrate recognition.Nucleic acids research · 2021Article
- Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus.Journal of microbiology (Seoul, Korea) · 2021Article
- Article
- Quantitative Control for Stoichiometric Protein Synthesis.Annual review of microbiology · 2021Article
- The effect of two ribonucleases on the production of Shiga toxin and stx-bearing bacteriophages in Enterohaemorrhagic Escherichia coli.Scientific reports · 2021Article
- Article
- A ribonuclease III involved in virulence of Mucorales fungi has evolved to cut exclusively single-stranded RNA.Nucleic acids research · 2021Article
15 more citing papers are in PubMed but not listed here.
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9 authors.
Funding
Abstract
Tiling microarrays have proven to be a valuable tool for gaining insights into the transcriptomes of microbial organisms grown under various nutritional or stress conditions. Here, we describe the use of such an array, constructed at the level of 20 nt resolution for the Escherichia coli MG1655 genome, to observe genome-wide changes in the steady-state RNA levels in mutants defective in either RNase E or RNase III. The array data were validated by comparison to previously published results for a variety of specific transcripts as well as independent northern analysis of additional mRNAs and sRNAs. In the absence of RNase E, 60% of the annotated coding sequences showed either increases or decreases in their steady-state levels. In contrast, only 12% of the coding sequences were affected in the absence of RNase III. Unexpectedly, many coding sequences showed decreased abundance in the RNase E mutant, while more than half of the annotated sRNAs showed changes in abundance. Furthermore, the steady-state levels of many transcripts showed overlapping effects of both ribonucleases. Data are also presented demonstrating how the arrays were used to identify potential new genes, RNase III cleavage sites and the direct or indirect control of specific biological pathways.
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