Evidence map›Paper›PMID 21148742›Full record

ArticleAmerican journal of respiratory cell and molecular biology2011

JUN-CCAAT/enhancer-binding protein complexes inhibit surfactant-associated protein B promoter activity.

Kiflai Bein, Hayley Leight, George D Leikauf

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of respiratory cell and molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.2field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. LPS-treated macrophage cytokines repress surfactant protein-B in lung epithelial cells.American journal of respiratory cell and molecular biology · 2013
    Article
  9. Article
  10. Article
  11. Integrative assessment of chlorine-induced acute lung injury in mice.American journal of respiratory cell and molecular biology · 2012
    Article
  12. Haplotype association mapping of acute lung injury in mice implicates activin a receptor, type 1.American journal of respiratory and critical care medicine · 2011
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Kiflai BeinDepartment of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, PA 15219-3130, USA. Kbein@pitt.edu
Hayley Leight
George D Leikauf
University of Pittsburgh · US

Funding

Improving our mechanistic understanding of Electronic-cigarette, or vaping, product use-associated lung injuryU01ES015675 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI LEIKAUF, GEORGE DOUGLAS · 2006 to 2020
$8.9M
Growth Factor Protection in Acute Lung InjuryR01HL077763 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI LEIKAUF, GEORGE DOUGLAS · 2006 to 2009
$1.4M
Role of Metalloproteinases in Mucin Overproduction in COPDR01HL085655 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI LEIKAUF, GEORGE DOUGLAS · 2008 to 2011
$1.4M
NHLBI NIH HHS HL077763NHLBI NIH HHS HL085655NHLBI NIH HHS R01 HL085655NIEHS NIH HHS ES015675NIEHS NIH HHS U01 ES015675
6 · The paper itself

Abstract

The murine surfactant-associated protein B (Sftpb) gene promoter, spanning nucleotides -653 to +42, is composed of functionally distinct proximal and distal regions. Although both regions contain consensus/putative activator protein 1 (AP-1) sites, the distal, but not the proximal, region mediates the inhibition by jun proto-oncogene (JUN) of Sftpb promoter activity. In transient cotransfection assays, JUN inhibited the luciferase reporter activity of plasmid constructs containing Sftpb promoter fragments that lacked the distal putative AP-1 site, indicating that another regulatory motif mediates JUN-dependent inhibition. Electrophoretic mobility shift assays and in silico analyses identified a DNA target sequence (Sftpb nucleotides -339 to -316) and transcription factors that regulate Sftpb promoter activity. The identified sequence contains a CCAAT/enhancer-binding protein (C/EBP) consensus recognition element. Mutation of the site reduced Sftpb promoter activity and sensitivity to inhibition by JUN. Purified recombinant JUN, which did not recognize the -339 to -316 target sequence when added alone, supershifted the mobility of in vitro translated C/EBP-α and C/EBP-β proteins complexed with the identified cis-regulatory element. These findings support the idea that heterodimerization between JUN and C/EBP-α and/or C/EBP-β targets JUN to the Sftpb promoter, thereby mediating its inhibitory regulatory role.

Indexed as

AnimalsBase SequenceBinding SitesCCAAT-Enhancer-Binding Protein-betaCCAAT-Enhancer-Binding ProteinsMiceMolecular Sequence DataMutationPromoter Regions, GeneticProtein BindingProto-Oncogene Proteins c-junPulmonary Surfactant-Associated Protein BRegulatory Sequences, Nucleic AcidSequence Homology, Nucleic AcidTranscriptional ActivationTranscription Factor AP-1CCAAT-Enhancer-Binding Protein-betaCCAAT-Enhancer-Binding ProteinsCEBPA protein, mouseCebpb protein, mouseProto-Oncogene Proteins c-junPulmonary Surfactant-Associated Protein BTranscription Factor AP-1

Identifiers

PMID21148742
PMCPMC3175569
OpenAlexW2155648139

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.