ArticleJournal of bacteriology2011
Genome-wide identification of Mycobacterium tuberculosis exported proteins with roles in intracellular growth.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed.
- Molecular Cloning, Purification and Characterization of Mce1R of Mycobacterium tuberculosis.Molecular biotechnology · 2021Article
- Metabolic Versatility ofMetabolites · 2021Review
- Early cell-autonomous accumulation of neutral lipids during infection promotes mycobacterial growth.PloS one · 2020Article
- Screening Mycobacterium tuberculosis Secreted Proteins Identifies Mpt64 as a Eukaryotic Membrane-Binding Bacterial Effector.mSphere · 2019Article
- Computational Identification of the Proteins Associated With Quorum Sensing and Biofilm Formation inFrontiers in microbiology · 2019Article
- Compartment-Specific Labeling of Bacterial Periplasmic Proteins by Peroxidase-Mediated Biotinylation.ACS infectious diseases · 2018Article
- Cholesterol and fatty acids grease the wheels of Mycobacterium tuberculosis pathogenesis.Pathogens and disease · 2018Review
- The Voltage-Dependent Anion Channels (VDAC) of Mycobacterium avium phagosome are associated with bacterial survival and lipid export in macrophages.Scientific reports · 2017Article
- The EXIT Strategy: an Approach for Identifying Bacterial Proteins Exported during Host Infection.mBio · 2017Article
- An orphaned Mce-associated membrane protein of Mycobacterium tuberculosis is a virulence factor that stabilizes Mce transporters.Molecular microbiology · 2016Article
- Label-free Quantitative Proteomics Reveals a Role for the Mycobacterium tuberculosis SecA2 Pathway in Exporting Solute Binding Proteins and Mce Transporters to the Cell Wall.Molecular & cellular proteomics : MCP · 2015Article
- A novel ESX-1 locus reveals that surface-associated ESX-1 substrates mediate virulence in Mycobacterium marinum.Journal of bacteriology · 2014Article
- Disconnecting in vitro ESX-1 secretion from mycobacterial virulence.Journal of bacteriology · 2013Article
- Rv2969c, essential for optimal growth in Mycobacterium tuberculosis, is a DsbA-like enzyme that interacts with VKOR-derived peptides and has atypical features of DsbA-like disulfide oxidases.Acta crystallographica. Section D, Biological crystallography · 2013Article
- Virulence factors of the Mycobacterium tuberculosis complex.Virulence · 2013Review
- Article
- The oxidation-sensing regulator (MosR) is a new redox-dependent transcription factor in Mycobacterium tuberculosis.The Journal of biological chemistry · 2012Article
- Direct detection of bacterial protein secretion using whole colony proteomics.Molecular & cellular proteomics : MCP · 2012Article
- The Mycobacterium tuberculosis SecA2 system subverts phagosome maturation to promote growth in macrophages.Infection and immunity · 2012Article
- The ins and outs of Mycobacterium tuberculosis protein export.Tuberculosis (Edinburgh, Scotland) · 2012Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The exported proteins of Mycobacterium tuberculosis that are localized at the bacterial cell surface or secreted into the environment are ideally situated to interact with host factors and to function in virulence. In this study, we constructed a novel β-lactamase reporter transposon and used it directly in M. tuberculosis for genome-wide identification of exported proteins. From 177 β-lactam-resistant transposon mutants, we identified 111 different exported proteins. The majority of these proteins have no known function, and for nearly half of the proteins, our demonstration that they are exported when fused to a β-lactamase reporter is the first experimental proof of their extracytoplasmic localization. The transposon mutants in our banked library were of further value as a collection of mutants lacking individual exported proteins. By individually testing each of 111 mutants for growth in macrophages, six attenuated mutants with insertions in mce1A, mce1B, mce2F, rv0199, ctaC, and lppX were identified. Given that much of the M. tuberculosis genome encodes proteins of unknown function, our library of mapped transposon mutants is a valuable resource for efforts in functional genomics. This work also demonstrates the power of a β-lactamase reporter transposon that could be applied similarly to other bacterial pathogens.
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