ArticleJournal of bacteriology2011
FAD binding by ApbE protein from Salmonella enterica: a new class of FAD-binding proteins.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Flavin transferase ApbE: From discovery to applications.The Journal of biological chemistry · 2025Review
- Functional Characterization of RseC in the SoxR Reducing System and Its Role in Oxidative Stress Response inJournal of microbiology and biotechnology · 2024Article
- Characterization of components of a reducing system for SoxR in the cytoplasmic membrane of Escherichia coli.Journal of microbiology (Seoul, Korea) · 2022Article
- Article
- Conserved residue His-257 ofThe Journal of biological chemistry · 2019Article
- Is there a Function for a Sex Pheromone Precursor?Journal of integrative bioinformatics · 2019Article
- Crystal structure of an assembly intermediate of respiratory Complex II.Nature communications · 2018Article
- The Membrane-Bound C Subunit of Reductive Dehalogenases: Topology Analysis and Reconstitution of the FMN-Binding Domain of PceC.Frontiers in microbiology · 2018Article
- Genome scale identification, structural analysis, and classification of periplasmic binding proteins from Mycobacterium tuberculosis.Current genetics · 2017Article
- Kinetic characterization of Vibrio cholerae ApbE: Substrate specificity and regulatory mechanisms.PloS one · 2017Article
- Efficient Sulfide Assimilation in Methanosarcina acetivorans Is Mediated by the MA1715 Protein.Journal of bacteriology · 2016Article
- Molecular insights into the enzymatic diversity of flavin-trafficking protein (Ftp; formerly ApbE) in flavoprotein biogenesis in the bacterial periplasm.MicrobiologyOpen · 2016Article
- Article
- Exploring the electron transfer pathway in the oxidation of avermectin by CYP107Z13 in Streptomyces ahygroscopicus ZB01.PloS one · 2014Article
- Variable suites of non-effector genes are co-regulated in the type III secretion virulence regulon across the Pseudomonas syringae phylogeny.PLoS pathogens · 2014Article
- ubiI, a new gene in Escherichia coli coenzyme Q biosynthesis, is involved in aerobic C5-hydroxylation.The Journal of biological chemistry · 2013Article
- Alternative pyrimidine biosynthesis protein ApbE is a flavin transferase catalyzing covalent attachment of FMN to a threonine residue in bacterial flavoproteins.The Journal of biological chemistry · 2013Article
- The TP0796 lipoprotein of Treponema pallidum is a bimetal-dependent FAD pyrophosphatase with a potential role in flavin homeostasis.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The periplasmic protein ApbE was identified through the analysis of several mutants defective in thiamine biosynthesis and was implicated as having a role in iron-sulfur cluster biosynthesis or repair. While mutations in apbE cause decreased activity of several iron-sulfur enzymes in vivo, the specific role of ApbE remains unknown. Members of the AbpE family include NosX and RnfF, which have been implicated in oxidation-reduction associated with nitrous oxide and nitrogen metabolism, respectively. In this work, we show that ApbE binds one FAD molecule per monomeric unit. The structure of ApbE in the presence of bound FAD reveals a new FAD-binding motif. Protein variants that are nonfunctional in vivo were generated by random and targeted mutagenesis. Each variant was substituted in the environment of the FAD and analyzed for FAD binding after reconstitution. The variant that altered a key tyrosine residue involved in FAD binding prevented reconstitution of the protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.