ArticleApplied and environmental microbiology2011
The cell membrane as a major site of damage during aerosolization of Escherichia coli.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Advancing transcriptomic profiling of airborne bacteria.Applied and environmental microbiology · 2025Review
- Viability studies of bacterial strains exposed to nitrogen oxides and light in controlled atmospheric conditions.Scientific reports · 2025Article
- The atmosphere: a transport medium or an active microbial ecosystem?The ISME journal · 2024Review
- Complete reference genome assemblies and annotations of threeMicrobiology resource announcements · 2023Article
- Article
- Utility of Three Nebulizers in Investigating the Infectivity of Airborne Viruses.Applied and environmental microbiology · 2021Article
- Aerosol agitation: Quantifying the hydrodynamic stressors on particulates encapsulated in small droplets.Physical review fluids · 2021Article
- Transformative Approach To Investigate the Microphysical Factors Influencing Airborne Transmission of Pathogens.Applied and environmental microbiology · 2020Article
- A Vibrating Mesh Nebulizer as an Alternative to the Collison Three-Jet Nebulizer for Infectious Disease Aerobiology.Applied and environmental microbiology · 2019Article
- Assessing the airborne survival of bacteria in populations of aerosol droplets with a novel technology.Journal of the Royal Society, Interface · 2019Article
- Optimisation of the Protocol for the LIVE/DEADFrontiers in microbiology · 2019Article
- Aerobiological Stabilities of Different Species of Gram-Negative Bacteria, Including Well-Known Biothreat Simulants, in Single-Cell Particles and Cell Clusters of Different Compositions.Applied and environmental microbiology · 2017Article
- Aerobiology: Experimental Considerations, Observations, and Future Tools.Applied and environmental microbiology · 2017Review
- Impact of serotype and sequence type on the preferential aerosolization of Streptococcus suis.BMC research notes · 2016Article
- Application of ATP-based bioluminescence for bioaerosol quantification: effect of sampling method.Journal of aerosol science · 2015Article
- Characterization and application of a nose-only exposure chamber for inhalation delivery of liposomal drugs and nucleic acids to mice.Journal of aerosol medicine and pulmonary drug delivery · 2013Article
- Release of free DNA by membrane-impaired bacterial aerosols due to aerosolization and air sampling.Applied and environmental microbiology · 2013Article
- Evaluation of microbiological air quality and of microclimate in university classrooms.Environmental monitoring and assessment · 2012Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to provide data on the survival and site of damage of Escherichia coli cells following aerosolization using two different techniques, nebulization and flow focusing. Four metabolic stains were assessed for their ability to detect respiratory activities and membrane homeostasis in aerosolized E. coli cells. The degree of sublethal injury increased significantly over the 10-min period of aerosolization in E. coli cells aerosolized by using the Collison nebulizer, reaching up to 99.9% of the population. In contrast, a significantly lower proportion of the population was sublethally damaged during aerosolization using the flow-focusing aerosol generator (FFAG). Concomitantly, loss of membrane homeostasis increased at a higher rate in nebulized cells (68 to 71%) than in those aerosolized by using the FFAG (32 to 34%). The activities of respiratory enzymes decreased at increased rates in nebulized cells (27 to 37%) compared to the rates of decrease in cells aerosolized by using the FFAG (59 to 61%). The results indicate that the physiology of an aerosolized bacterium is linked to the method of aerosol generation and may affect the interpretation of a range of aerobiological phenomenon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.