ArticleApplied and environmental microbiology2011
Among developmental regulators, StuA but not BrlA is essential for penicillin V production in Penicillium chrysogenum.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 52 citations in OpenAlex.
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- Characterization and engineering of the xylose-inducibleMetabolic engineering communications · 2022Article
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- Beyond the Biosynthetic Gene Cluster Paradigm: Genome-Wide Coexpression Networks Connect Clustered and Unclustered Transcription Factors to Secondary Metabolic Pathways.Microbiology spectrum · 2021Article
- Regulation of Secondary Metabolism in theInternational journal of molecular sciences · 2020Review
- TheInternational journal of molecular sciences · 2020Article
- Antifungal Peptides of the AFP Family Revisited: Are These Cannibal Toxins?Microorganisms · 2018Review
- Dicer and Argonaute Genes Involved in RNA Interference in the Entomopathogenic Fungus Metarhizium robertsii.Applied and environmental microbiology · 2017Article
- The transcriptional regulators SteA and StuA contribute to keratin degradation and sexual reproduction of the dermatophyte Arthroderma benhamiae.Current genetics · 2017Article
- A Transcriptome Meta-Analysis Proposes Novel Biological Roles for the Antifungal Protein AnAFP in Aspergillus niger.PloS one · 2016Article
- Article
- Phylogeny, identification and nomenclature of the genus Aspergillus.Studies in mycology · 2014Article
- A novel C2H2 transcription factor that regulates gliA expression interdependently with GliZ in Aspergillus fumigatus.PLoS genetics · 2014Article
- WetA is required for conidiogenesis and conidium maturation in the ascomycete fungus Fusarium graminearum.Eukaryotic cell · 2014Article
- Proteomics shows new faces for the old penicillin producer Penicillium chrysogenum.Journal of biomedicine & biotechnology · 2012Review
- Transcriptional regulatory elements in fungal secondary metabolism.Journal of microbiology (Seoul, Korea) · 2011Review
- The paf gene product modulates asexual development in Penicillium chrysogenum.Journal of basic microbiology · 2011Article
- Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In filamentous fungi, secondary metabolism is often linked with developmental processes such as conidiation. In this study we analyzed the link between secondary metabolism and conidiation in the main industrial producer of the β-lactam antibiotic penicillin, the ascomycete Penicillium chrysogenum. Therefore, we generated mutants defective in two central regulators of conidiation, the transcription factors BrlA and StuA. Inactivation of either brlA or stuA blocked conidiation and altered hyphal morphology during growth on solid media, as shown by light and scanning electron microscopy, but did not affect biomass production during liquid-submerged growth. Genome-wide transcriptional profiling identified a complex StuA- and BrlA-dependent regulatory network, including genes previously shown to be involved in development and secondary metabolism. Remarkably, inactivation of stuA, but not brlA, drastically downregulated expression of the penicillin biosynthetic gene cluster during solid and liquid-submerged growth. In agreement, penicillin V production was wild-type-like in brlA-deficient strains but 99% decreased in stuA-deficient strains during liquid-submerged growth, as shown by high-performance liquid chromatography (HPLC) analysis. Thus, among identified regulators of penicillin V production StuA has the most severe influence. Overexpression of stuA increased the transcript levels of brlA and abaA (another developmental regulator) and derepressed conidiation during liquid-submerged growth but did not affect penicillin V productivity. Taken together, these data demonstrate an intimate but not exclusive link between regulation of development and secondary metabolism in P. chrysogenum.
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