ArticlePlant physiology2011
Hemin and magnesium-protoporphyrin IX induce global changes in gene expression in Chlamydomonas reinhardtii.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Regulatory and retrograde signaling networks in the chlorophyll biosynthetic pathway.Journal of integrative plant biology · 2025Review
- Progress in Research on the Mechanisms Underlying Chloroplast-Involved Heat Tolerance in Plants.Genes · 2021Review
- GUN control in retrograde signaling: How GENOMES UNCOUPLED proteins adjust nuclear gene expression to plastid biogenesis.The Plant cell · 2021Review
- The Role of Tetrapyrrole- and GUN1-Dependent Signaling on Chloroplast Biogenesis.Plants (Basel, Switzerland) · 2021Review
- Proteomic analysis of haem-binding protein fromPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Article
- Omics Application of Bio-Hydrogen Production Through Green AlgaFrontiers in bioengineering and biotechnology · 2019Review
- Genome Analyses of the Microalga Picochlorum Provide Insights into the Evolution of Thermotolerance in the Green Lineage.Genome biology and evolution · 2018Article
- An Overview of Biomembrane Functions in Plant Responses to High-Temperature Stress.Frontiers in plant science · 2018Review
- An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii.The Plant cell · 2016Article
- Chloroplast Retrograde Regulation of Heat Stress Responses in Plants.Frontiers in plant science · 2016Review
- Tetrapyrrole Signaling in Plants.Frontiers in plant science · 2016Review
- Identification of novel small ncRNAs in pollen of tomato.BMC genomics · 2015Article
- Chloroplast Biogenesis-Associated Nuclear Genes: Control by Plastid Signals Evolved Prior to Their Regulation as Part of Photomorphogenesis.Frontiers in plant science · 2015Article
- Increased expression of Fe-chelatase leads to increased metabolic flux into heme and confers protection against photodynamically induced oxidative stress.Plant molecular biology · 2014Article
- Retrograde bilin signaling enables Chlamydomonas greening and phototrophic survival.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A model for tetrapyrrole synthesis as the primary mechanism for plastid-to-nucleus signaling during chloroplast biogenesis.Frontiers in plant science · 2013Article
- The PSBP2 protein of Chlamydomonas reinhardtii is required for singlet oxygen-dependent signaling.Planta · 2012Article
- Reconsidering the nature and mode of action of metabolite retrograde signals from the chloroplast.Frontiers in plant science · 2012Article
- Construction and evaluation of a whole genome microarray of Chlamydomonas reinhardtii.BMC genomics · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retrograde signaling is a pathway of communication from mitochondria and plastids to the nucleus in the context of cell differentiation, development, and stress response. In Chlamydomonas reinhardtii, the tetrapyrroles magnesium-protoporphyrin IX and heme are only synthesized within the chloroplast, and they have been implicated in the retrograde control of nuclear gene expression in this unicellular green alga. Feeding the two tetrapyrroles to Chlamydomonas cultures was previously shown to transiently induce five nuclear genes, three of which encode the heat shock proteins HSP70A, HSP70B, and HSP70E. In contrast, controversial results exist on the possible role of magnesium-protoporphyrin IX in the repression of genes for light-harvesting proteins in higher plants, raising the question of how important this mode of regulation is. Here, we used genome-wide transcriptional profiling to measure the global impact of these tetrapyrroles on gene regulation and the scope of the response. We identified almost 1,000 genes whose expression level changed transiently but significantly. Among them were only a few genes for photosynthetic proteins but several encoding enzymes of the tricarboxylic acid cycle, heme-binding proteins, stress-response proteins, as well as proteins involved in protein folding and degradation. More than 50% of the latter class of genes was also regulated by heat shock. The observed drastic fold changes at the RNA level did not correlate with similar changes in protein concentrations under the tested experimental conditions. Phylogenetic profiling revealed that genes of putative endosymbiontic origin are not overrepresented among the responding genes. This and the transient nature of changes in gene expression suggest a signaling role of both tetrapyrroles as secondary messengers for adaptive responses affecting the entire cell and not only organellar proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.