ArticleInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases2011
High-throughput molecular diagnosis of circumsporozoite variants VK210 and VK247 detects complex Plasmodium vivax infections in malaria endemic populations in Papua New Guinea.
Article in Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 19 citations in OpenAlex.
- Genetic signatures of Plasmodium vivax Circumsporozoite Surface Protein during malaria resurgence in Thailand.International journal for parasitology · 2026Article
- Genetic Diversity of Circumsporozoite Surface Protein ofPathogens (Basel, Switzerland) · 2022Article
- Advancing Research Models and Technologies to Overcome Biological Barriers to Plasmodium vivax Control.Trends in parasitology · 2018Review
- Serological markers to measure recent changes in malaria at population level in Cambodia.Malaria journal · 2016Article
- Implementation and application of a multiplex assay to detect malaria-specific antibodies: a promising tool for assessing malaria transmission in Southeast Asian pre-elimination areas.Malaria journal · 2015Article
- The utility of genomic data for Plasmodium vivax population surveillance.Pathogens and global health · 2015Review
- Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes.PLoS neglected tropical diseases · 2015Article
- Genetic diversity of Plasmodium Vivax in South of Iran: A cross-sectional study.Journal of medicine and life · 2015Article
- Differing patterns of selection and geospatial genetic diversity within two leading Plasmodium vivax candidate vaccine antigens.PLoS neglected tropical diseases · 2014Article
- Serological responses to a soluble recombinant chimeric Plasmodium vivax circumsporozoite protein in VK210 and VK247 population.Malaria journal · 2013Article
- High genetic diversity of Plasmodium vivax on the north coast of Papua New Guinea.The American journal of tropical medicine and hygiene · 2013Article
- Understanding the population genetics of Plasmodium vivax is essential for malaria control and elimination.Malaria journal · 2012Review
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Malaria is endemic in lowland and coastal regions of Papua New Guinea (PNG), and is caused by Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale. Infection by P. vivax is attributed to distinct strains, VK210 and VK247, which differ in the sequence of the circumsporozoite protein (pvcsp). Here, based upon sequence polymorphisms in pvcsp, we developed a post-PCR ligation detection reaction-fluorescent microsphere assay (LDR-FMA) to distinguish these P. vivax strains. This diagnostic assay was designed to detect the presence of both VK210 and VK247 P. vivax strains simultaneously in a high-throughput 96-well format. Using this assay, we analyzed human blood samples from the Wosera (n=703) and Mugil (n=986) regions to evaluate the prevalence of these P. vivax strains. VK210 and VK247 strains were found in both study sites. In the Wosera, single infections with VK210 strain were observed to be most common (41.7%), followed by mixed-strain (36.8%) and VK247 single-strain infections (21.5%). Similarly, in Mugil, VK210 single-strain infections were most common (51.6%), followed by mixed-strain (34.4%) and VK247 single-strain infections (14%). These results suggest that the distribution of P. vivax infections was similar between the two study sites. Interestingly, we observed a non-random distribution of these two P. vivax strains, as mixed-strain infections were significantly more prevalent than expected in both study sites (Wosera and Mugil χ(2)p-value<0.001). Additionally, DNA sequence analysis of a subset of P. vivax infections showed that no individual pvcsp alleles were shared between the two study sites. Overall, our results illustrate that PNG malaria-endemic regions harbor a complex mixture of P. vivax strains, and emphasize the importance of malaria control strategies that would be effective against a highly diverse parasite population.
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