ArticleDevelopmental biology2011
mitfa is required at multiple stages of melanocyte differentiation but not to establish the melanocyte stem cell.
Article in Developmental biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 92 citations in OpenAlex.
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- Loss of MITF activity leads to emergent cell states from the melanocyte stem cell lineage.bioRxiv : the preprint server for biology · 2026Article
- Dominant Negative Mitf Allele Impacts Melanophore and Xanthophore Development and Reveals Collaborative Interactions With Tfec in Zebrafish Chromatophore Lineages.Pigment cell & melanoma research · 2025Article
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- Intercross population study reveals that co-mutation ofZoological research · 2023Article
- MITF deficiency and oncogenic GNAQ each promote proliferation programs in zebrafish melanocyte lineage cells.Pigment cell & melanoma research · 2022Article
- Fate mapping melanoma persister cells through regression and into recurrent disease in adult zebrafish.Disease models & mechanisms · 2022Article
- Assessing the Morphological and Behavioral Toxicity of Catechol Using Larval Zebrafish.International journal of molecular sciences · 2022Article
- SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish.Nature communications · 2022Article
- Aldh2 is a lineage-specific metabolic gatekeeper in melanocyte stem cells.Development (Cambridge, England) · 2022Article
- Zebrafish Syndromic Albinism Models as Tools for Understanding and Treating Pigment Cell Disease in Humans.Cancers · 2022Review
- Tfap2b specifies an embryonic melanocyte stem cell that retains adult multifate potential.Cell reports · 2022Article
- Cell Fate Decisions in the Neural Crest, from Pigment Cell to Neural Development.International journal of molecular sciences · 2021Review
- Pigment pattern morphospace of Danio fishes: evolutionary diversification and mutational effects.Biology open · 2021Article
- Immunoglobulin superfamily receptor Junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes.Developmental biology · 2021Article
- Toward whole tissue imaging of axolotl regeneration.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Review
- In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning.Nature communications · 2020Article
- PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation.Developmental cell · 2020Article
- Neural innervation as a potential trigger of morphological color change and sexual dimorphism in cichlid fish.Scientific reports · 2020Article
- Developmental and Cellular Basis of Vertical Bar Color Patterns in the East African Cichlid FishFrontiers in cell and developmental biology · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The mitfa gene encodes a zebrafish ortholog of the microphthalmia-associated transcription factor (Mitf) which, like its counterparts in other species, is absolutely required for development of neural crest melanocytes. In order to evaluate mitfa's role in different stages of melanocyte development, we have identified hypomorphic alleles of mitfa, including two alleles that are temperature-sensitive for melanocyte development. Molecular analysis revealed that the mitf(fh53)ts results from a single base pair change producing an asparagine to tyrosine amino acid substitution in the DNA-binding domain, and the mitfa(vc7)ts allele is a mutation in a splice donor site that reduces the level of correctly-spliced transcripts. Splicing in the mitfa(vc7) allele does not itself appear to be temperature-dependent. A third, hypomorphic allele, mitfa(z25) results in an isoleucine to phenylalanine substitution in the first helix domain of the protein. Temperature upshift experiments with mitfa(fh53)ts show that mitfa is required at several stages of melanocyte differentiation, including for expression of the early melanoblast marker dct, again for progression from dct expression to differentiation, and again for maintenance of dendritic form following differentiation. mitfa(fh53)ts mutants recover melanocytes within 2-3days when downshifted at all stages of larval development. However, when melanocyte stem cells (MSCs) are ablated by early treatment with the erbB3 inhibitor AG1478, melanocyte recovery is lost by 48 h. This result indicates first that the MSC is established at the restrictive temperature, and that melanoblasts die or lose the ability to recover after being held at the restrictive temperature for approximately one day.
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