ArticleMolecular microbiology2010
The SAM-responsive S(MK) box is a reversible riboswitch.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- NusG-dependent RNA polymerase pausing is a common feature of riboswitch regulatory mechanisms.Nucleic acids research · 2024Article
- Fingerprinting Tertiary Structure in Complex RNAs Using Single-Molecule Correlated Chemical Probing.Biochemistry · 2024Article
- A transient intermediate RNA structure underlies the regulatory function of theRNA (New York, N.Y.) · 2023Article
- RNA-Ligand Interactions Quantified by Surface Plasmon Resonance with Reference Subtraction.Biochemistry · 2022Article
- Incoherent dual regulation by a SAM-II riboswitch controlling translation at a distance.RNA biology · 2022Article
- Naturally occurring three-way junctions can be repurposed as genetically encoded RNA-based sensors.Cell chemical biology · 2021Article
- Riboswitch Mechanisms: New Tricks for an Old Dog.Biochemistry. Biokhimiia · 2021Review
- Transcriptional and translational S-box riboswitches differ in ligand-binding properties.The Journal of biological chemistry · 2020Article
- Single-Nucleotide RNA Maps for the Two Major Nosocomial PathogensFrontiers in cellular and infection microbiology · 2020Article
- A Fluorogenic RNA-Based Sensor Activated by Metabolite-Induced RNA Dimerization.Cell chemical biology · 2019Article
- SAM-VI riboswitch structure and signature for ligand discrimination.Nature communications · 2019Article
- Life times of metastable states guide regulatory signaling in transcriptional riboswitches.Nature communications · 2018Article
- A magnesium-induced triplex pre-organizes the SAM-II riboswitch.PLoS computational biology · 2017Article
- Themes and variations in riboswitch structure and function.Biochimica et biophysica acta · 2014Review
- Common themes and differences in SAM recognition among SAM riboswitches.Biochimica et biophysica acta · 2014Review
- Comparative genomics of transcriptional regulation of methionine metabolism in Proteobacteria.PloS one · 2014Article
- Kinetics of allosteric transitions in S-adenosylmethionine riboswitch are accurately predicted from the folding landscape.Journal of the American Chemical Society · 2013Article
- Riboswitch control of induction of aminoglycoside resistance acetyl and adenyl-transferases.RNA biology · 2013Article
- Multiple conformations of SAM-II riboswitch detected with SAXS and NMR spectroscopy.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The S(MK) (SAM-III) box is an S-adenosylmethionine (SAM)-responsive riboswitch found in the 5' untranslated region of metK genes, encoding SAM synthetase, in many members of the Lactobacillales. SAM binding causes a structural rearrangement in the RNA that sequesters the Shine-Dalgarno (SD) sequence by pairing with a complementary anti-SD (ASD) sequence; sequestration of the SD sequence inhibits binding of the 30S ribosomal subunit and prevents translation initiation. We observed a slight increase in the half-life of the metK transcript in vivo when Enterococcus faecalis cells were depleted for SAM, but no significant change in overall transcript abundance, consistent with the model that this riboswitch regulates at the level of translation initiation. The half-life of the SAM-S(MK) box RNA complex in vitro is shorter than that of the metK transcript in vivo, raising the possibility of reversible binding of SAM. We used a fluorescence assay to directly visualize reversible switching between the SAM-free and SAM-bound conformations. We propose that the S(MK) box riboswitch can make multiple SAM-dependent regulatory decisions during the lifetime of the transcript in vivo, acting as a reversible switch that allows the cell to respond rapidly to fluctuations in SAM pools by modulating expression of the SAM synthetase gene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.