ArticleAnalytical chemistry2011
Multiplexed protein quantification with barcoded hydrogel microparticles.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed.
- Stimuli-Responsive Hydrogels: From Swelling-Deswelling Mechanisms to Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2026Review
- MIDAS: rapid, multiplexed molecular profiling for integrated host-pathogen analysis.Nature communications · 2025Article
- Tunable Picoliter-Scale Dropicle Formation Using Amphiphilic Microparticles with Patterned Hydrophilic Patches.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Squeezable Hydrogel Microparticles for Single Extracellular Vesicle Protein Profiling.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Lab on a Particle Technologies.Analytical chemistry · 2024Review
- Spatially Defined Cell-Secreted Protein Detection Using Granular Hydrogels: μGeLISA.ACS biomaterials science & engineering · 2023Article
- Gold-nanoparticle-embedded hydrogel droplets with enhanced fluorescence for imaging and quantification of proteins in cells.Mikrochimica acta · 2023Article
- Single-Color Barcoding for Multiplexed Hydrogel Bead-Based Immunoassays.ACS applied materials & interfaces · 2022Article
- Recent Advances in Polymer Additive Engineering for Diagnostic and Therapeutic Hydrogels.International journal of molecular sciences · 2022Review
- Electrotransfer of Immunoprobes through Thin-Layer Polyacrylamide Gels.Analytical chemistry · 2022Article
- Article
- Rapid, multiplexed detection of biomolecules using electrically distinct hydrogel beads.Lab on a chip · 2020Article
- Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins.Nature communications · 2020Article
- Article
- MRBLES 2.0: High-throughput generation of chemically functionalized spectrally and magnetically encoded hydrogel beads using a simple single-layer microfluidic device.Microsystems & nanoengineering · 2020Article
- Multiplexed in-gel microfluidic immunoassays: characterizing protein target loss during reprobing of benzophenone-modified hydrogels.Scientific reports · 2019Article
- Ultrasimple Single-Cell Detection of Multiple Cytokines by a Nanowell Chip Integrated with Encoded Microarrays.ACS sensors · 2019Article
- Low temperature flow lithography.Biomicrofluidics · 2018Article
- Quantitative and multiplex microRNA assays from unprocessed cells in isolated nanoliter well arrays.Lab on a chip · 2018Article
- Generation of Well-Defined Micro/Nanoparticles via Advanced Manufacturing Techniques for Therapeutic Delivery.Materials (Basel, Switzerland) · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We demonstrate the use of graphically encoded hydrogel microparticles for the sensitive and high-throughput multiplexed detection of clinically relevant protein panels in complex media. Combining established antibody capture techniques with advances in both microfluidic synthesis and analysis, we detected 1-8 pg/mL amounts of three cytokines (interleuken-2, interleuken-4, and tumor necrosis factor alpha) in single and multiplexed assays without the need for filtration or blocking agents. A range of hydrogel porosities was investigated to ensure rapid diffusion of targets and reagents into the particle as well as to maintain the structural integrity of particles during rinsing procedures and high-velocity microfluidic scanning. Covalent incorporation of capture antibodies using a heterobifunctional poly(ethylene glycol) linker enabled one-step synthesis and functionalization of particles using only small amounts of valuable reagents. In addition to the use of three separate types of single-probe particles, the flexibility of the stop-flow lithography (SFL) method was leveraged to spatially segregate the three probes for the aforementioned target set on an individual encoded particle, thereby demonstrating the feasibility of single-particle diagnostic panels. This study establishes the gel-particle platform as a versatile tool for the efficient quantification of protein targets and significantly advances efforts to extend the advantages of both hydrogel substrates and particle-based arrays to the field of clinical proteomics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.