ArticleBioconjugate chemistry2011
A versatile method for functionalizing surfaces with bioactive glycans.
Article in Bioconjugate chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- Investigation of a chondroitin sulfate-based bioactive coating for neural interface applications.Journal of materials chemistry. B · 2024Article
- Surface Functionalization Strategies of Polystyrene for the Development Peptide-Based Toxin Recognition.Sensors (Basel, Switzerland) · 2022Article
- Biofunctionalization of Graphene-Based FET Sensors through Heterobifunctional Nanoscaffolds: Technology Validation toward Rapid COVID-19 Diagnostics and Monitoring.Advanced materials interfaces · 2022Article
- High sensitivity analysis of nanogram quantities of glycosaminoglycans using ToF-SIMS.Communications chemistry · 2021Article
- π-Donor/π-Acceptor Interactions for the Encapsulation of Neurotransmitters on Functionalized Polysilicon-Based Microparticles.Pharmaceutics · 2020Article
- Achieving Controlled Biomolecule-Biomaterial Conjugation.Chemical reviews · 2018Review
- Subradiant Dipolar Interactions in Plasmonic Nanoring Resonator Array for Integrated Label-Free Biosensing.ACS sensors · 2017Article
- The versatility of N-alkyl-methoxyamine bi-functional linkers for the preparation of glycoconjugates.Glycoconjugate journal · 2017Article
- Poly(ethylene) glycol hydrogel based on oxa-Michael reaction: Precursor synthesis and hydrogel formation.Biointerphases · 2017Article
- Strategies toward protecting group-free glycosylation through selective activation of the anomeric center.Beilstein journal of organic chemistry · 2017Review
- ToF-SIMS analysis of a polymer microarray composed of poly(meth)acrylates with CSurface and interface analysis : SIA · 2016Article
- Inorganic materials as supports for covalent enzyme immobilization: methods and mechanisms.Molecules (Basel, Switzerland) · 2014Review
- Identifying human milk glycans that inhibit norovirus binding using surface plasmon resonance.Glycobiology · 2013Article
- Biofunctional paper via the covalent modification of cellulose.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- An organophosphonate strategy for functionalizing silicon photonic biosensors.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Use of fungal derived polysaccharide-conjugated particles to probe Dectin-1 responses in innate immunity.Integrative biology : quantitative biosciences from nano to macro · 2012Article
- Glycosylated self-assembled monolayers for arrays and surface analysis.Methods in molecular biology (Clifton, N.J.) · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Microarrays and biosensors owe their functionality to our ability to display surface-bound biomolecules with retained biological function. Versatile, stable, and facile methods for the immobilization of bioactive compounds on surfaces have expanded the application of high-throughput "omics"-scale screening of molecular interactions by nonexpert laboratories. Herein, we demonstrate the potential of simplified chemistries to fabricate a glycan microarray, utilizing divinyl sulfone (DVS)-modified surfaces for the covalent immobilization of natural and chemically derived carbohydrates, as well as glycoproteins. The bioactivity of the captured glycans was quantitatively examined by surface plasmon resonance imaging (SPRi). Composition and spectroscopic evidence of carbohydrate species on the DVS-modified surface were obtained by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS), respectively. The site-selective immobilization of glycans based on relative nucleophilicity (reducing sugar vs amine- and sulfhydryl-derived saccharides) and anomeric configuration was also examined. Our results demonstrate straightforward and reproducible conjugation of a variety of functional biomolecules onto a vinyl sulfone-modified biosensor surface. The simplicity of this method will have a significant impact on glycomics research, as it expands the ability of nonsynthetic laboratories to rapidly construct functional glycan microarrays and quantitative biosensors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.