ArticleMolecular vision2010
Gene expression analysis in SV-40 immortalized human corneal epithelial cells cultured with an air-liquid interface.
Article in Molecular vision, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- On the Misuse of Virus-Transformed Human Corneal Epithelial Cells as Surrogates for Normal Cells.Investigative ophthalmology & visual science · 2026Review
- Development and Validation of an In Vitro Ocular Irritation Test for Ophthalmic Medical Devices with a Novel Reconstructed Human Corneal Epithelium Model.Bioengineering (Basel, Switzerland) · 2026Article
- Glucosamine mitigates hyperglycemic-induced oxidative stress via the SIRT1 pathway in human corneal epithelial cells.Scientific reports · 2025Article
- Article
- The Biocompatibility Analysis of Artificial Mucin-Like Glycopolymers.International journal of molecular sciences · 2023Article
- Cell Cultures at the Air-Liquid Interface and Their Application in Cancer Research.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell-derived alveolar epithelium.JCI insight · 2022Article
- Expression of immune response genes in human corneal epithelial cells interacting with Aspergillus flavus conidia.BMC genomics · 2022Article
- Corneal Infection Models: Tools to Investigate the Role of Biofilms in Bacterial Keratitis.Cells · 2020Review
- HCE-T cell line lacks cornea-specific differentiation markers compared to primary limbal epithelial cells and differentiated corneal epithelium.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2020Article
- Effective In Vivo Topical Delivery of siRNA and Gene Silencing in Intact Corneal Epithelium Using a Modified Cell-Penetrating Peptide.Molecular therapy. Nucleic acids · 2019Article
- Identification of pathogenic genes of pterygium based on the Gene Expression Omnibus database.International journal of ophthalmology · 2019Article
- Matrix regeneration agents improve wound healing in non-stressed human corneal epithelial cells.Eye (London, England) · 2018Article
- Human corneal cell culture models for drug toxicity studies.Drug delivery and translational research · 2016Review
- Glucocorticoid action in human corneal epithelial cells establishes roles for corticosteroids in wound healing and barrier function of the eye.Experimental eye research · 2016Article
- Karyotype alteration generates the neoplastic phenotypes of SV40-infected human and rodent cells.Molecular cytogenetics · 2015Article
- Development of a curved, stratified, in vitro model to assess ocular biocompatibility.PloS one · 2014Article
- Porcine corneal cell culture models for studying epidemic keratoconjunctivitis.Molecular vision · 2013Article
- Role of CTCF in EGF-induced migration of immortalized human corneal epithelial cells.Investigative ophthalmology & visual science · 2012Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeTo compare the global gene expression profile of stratified epithelia generated in vitro using simian virus 40 (SV40) immortalized human corneal epithelial cells with the previously reported gene expression of normal human corneal epithelia.
methodsImmortalized cells expanded in submerged culture were grown in an air-liquid interface of liquid permeable collagen-coated filters to foster stratification and differentiation. Stratified epithelia displaying resistances exceeding 300 Ω·cm2 were dissolved in an RNA purification lysis buffer. Purified RNA was used to globally determine gene expression levels using high-density single-channel oligonucleotide microarrays. Raw hybridization readings were converted into relative gene expression levels using Robust Multi-array Average (RMA) algorithm. Expression levels for selected genes were validated by real-time RT-qPCR. The biologic significance of the gene expression profiles was interpreted with the help of several microarray software analysis tools and ad hoc thematical analysis.
resultsThe stratified cell culture to native epithelial comparison identified over- and under-expression in 22% and 14% of the probed genes, respectively. The larger expression decreases occurred in genes intimately associated with both the stratified epithelial lineage at large such as keratin 14 and the corneal phenotype, such as keratin 12, connexin 43, aldehyde dehydrogenases (ALDHs), and paired box gene 6 (PAX6) and its whole downstream transcriptome. Overexpression related to genes associated with cell cycling stimulation.
conclusionsThe results indicate that the stratified corneal epithelial cell model generated using SV40 immortalized cells may be useful only in certain research applications. Extrapolations of studies with these cells to actual tissue cells should be done with a great deal of caution.
Indexed as
Identifiers
21139686PMC2994346W2155841673What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.