ArticleThe EMBO journal2011
A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion.
Article in The EMBO journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 85 citations in OpenAlex.
- The SMC Hinge is a Selective Gate for Obstacle Bypass.Nature communications · 2025Article
- The Phenotype ofGenes · 2025Article
- Nucleic Acids and Electrical Signals.Reviews of physiology, biochemistry and pharmacology · 2025Review
- Article
- DNA tension-modulated translocation and loop extrusion by SMC complexes revealed by molecular dynamics simulations.Nucleic acids research · 2022Article
- Article
- Folding of cohesin's coiled coil is important for Scc2/4-induced association with chromosomes.eLife · 2021Article
- Nse5/6 is a negative regulator of the ATPase activity of the Smc5/6 complex.Nucleic acids research · 2021Article
- A cohesin cancer mutation reveals a role for the hinge domain in genome organization and gene expression.PLoS genetics · 2021Article
- Structural insights into DNA loop extrusion by SMC protein complexes.Current opinion in structural biology · 2020Review
- Comprehensive classification of ABC ATPases and their functional radiation in nucleoprotein dynamics and biological conflict systems.Nucleic acids research · 2020Review
- Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA.Molecular cell · 2020Article
- Isolation of Fission Yeast Condensin Temperature-Sensitive Mutants with Single Amino Acid Substitutions Targeted to Hinge Domain.G3 (Bethesda, Md.) · 2019Article
- Towards a Unified Model of SMC Complex Function.Current biology : CB · 2018Review
- Cohesin dynamic association to chromatin and interfacing with replication forks in genome integrity maintenance.Current genetics · 2018Review
- Article
- Suppressor mutation analysis combined with 3D modeling explains cohesin's capacity to hold and release DNA.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A role for the Smc3 hinge domain in the maintenance of sister chromatid cohesion.Molecular biology of the cell · 2018Article
- Cohesin SA2 is a sequence-independent DNA-binding protein that recognizes DNA replication and repair intermediates.The Journal of biological chemistry · 2018Article
- The torments of the cohesin ring.Nucleus (Austin, Tex.) · 2017Review
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 3 countries.
Funding
Abstract
Cohesin's structural maintenance of chromosome 1 (Smc1) and Smc3 are rod-shaped proteins with 50-nm long intra-molecular coiled-coil arms with a heterodimerization domain at one end and an ABC-like nucleotide-binding domain (NBD) at the other. Heterodimerization creates V-shaped molecules with a hinge at their centre. Inter-connection of NBDs by Scc1 creates a tripartite ring within which, it is proposed, sister DNAs are entrapped. To investigate whether cohesin's hinge functions as a possible DNA entry gate, we solved the crystal structure of the hinge from Mus musculus, which like its bacterial counterpart is characterized by a pseudo symmetric heterodimeric torus containing a small channel that is positively charged. Mutations in yeast Smc1 and Smc3 that together neutralize the channel's charge have little effect on dimerization or association with chromosomes, but are nevertheless lethal. Our finding that neutralization reduces acetylation of Smc3, which normally occurs during replication and is essential for cohesion, suggests that the positively charged channel is involved in a major conformational change during S phase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.