ArticleNeuro-oncology2011
Homozygous loss of ADAM3A revealed by genome-wide analysis of pediatric high-grade glioma and diffuse intrinsic pontine gliomas.
Article in Neuro-oncology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers, 1 of them a synthesis that pooled it.
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Who cites it
66 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Integrated Molecular Meta-Analysis of 1,000 Pediatric High-Grade and Diffuse Intrinsic Pontine Glioma.Cancer cell · 2017Pooled it
- Identification of genetic fingerprint of type I interferon therapy in visceral metastases of melanoma.Scientific reports · 2024Article
- DNA Methylation Profiles Are Stable in H3 K27M-Mutant Diffuse Midline Glioma Neurosphere Cell Lines.Children (Basel, Switzerland) · 2024Article
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- Epigenomic landscape and 3D genome structure in pediatric high-grade glioma.Science advances · 2021Article
- Mesenchymal Epithelial Transition Factor Signaling in Pediatric Nervous System Tumors: Implications for Malignancy and Cancer Stem Cell Enrichment.Frontiers in cell and developmental biology · 2021Review
- Evolution from adherent to suspension: systems biology of HEK293 cell line development.Scientific reports · 2020Article
- High-grade gliomas in adolescents and young adults highlight histomolecular differences from their adult and pediatric counterparts.Neuro-oncology · 2020Article
- Identification of prognostic markers in diffuse midline gliomas H3K27M-mutant.Brain pathology (Zurich, Switzerland) · 2020Article
- ADAM3A copy number gains occur in a subset of conjunctival squamous cell carcinoma and its high grade precursors.Human pathology · 2019Article
- Whole-exome sequencing revealed mutational profiles of giant cell glioblastomas.Brain pathology (Zurich, Switzerland) · 2019Article
- Review
- Glioblastoma spheroids produce infiltrative gliomas in the rat brainstem.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2017Article
- Application of high-resolution genomic profiling in the differential diagnosis of liposarcoma.Molecular cytogenetics · 2017Article
- Potential New Therapies for Pediatric Diffuse Intrinsic Pontine Glioma.Frontiers in pharmacology · 2017Review
- Convection-Enhanced Delivery for Diffuse Intrinsic Pontine Glioma Treatment.Current neuropharmacology · 2017Article
- Re-Examining the Need for Tissue Diagnosis in Pediatric Diffuse Intrinsic Pontine Gliomas: A Review.Current neuropharmacology · 2017Review
- Diffuse Intrinsic Pontine Glioma: Time for Cautious Optimism.Journal of child neurology · 2016Review
- Passenger mutations and aberrant gene expression in congenic tissue plasminogen activator-deficient mouse strains.Journal of thrombosis and haemostasis : JTH · 2016Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Overall, pediatric high-grade glioma (pHGG) has a poor prognosis, in part due to the lack of understanding of the underlying biology. High-resolution 244 K oligo array comparative genomic hybridization (CGH) was used to analyze DNA from 38 formalin-fixed paraffin-embedded predominantly pretreatment pHGG samples, including 13 diffuse intrinsic pontine gliomas (DIPGs). The patterns of gains and losses were distinct from those seen in HGG arising in adults. In particular, we found 1q gain in up to 27% of our cohort compared with 9% reported in adults. A total of 13% had a balanced genetic profile with no large-scale copy number alterations. Homozygous loss at 8p12 was seen in 6 of 38 (16%) cases of pHGG. This novel deletion, which includes the ADAM3A gene, was confirmed by quantitative real-time PCR (qPCR). Loss of CDKN2A/CDKN2B in 4 of 38 (10%) samples by oligo array CGH was confirmed by fluorescent in situ hybridization on tissue microarrays and was restricted to supratentorial tumors. Only ∼50% of supratentorial tumors were positive for CDKN2B expression by immunohistochemistry (IHC), while ∼75% of infratentorial tumors were positive for CDKN2B expression (P = 0.03). Amplification of the 4q11-13 region was detected in 8% of cases and included PDGFRA and KIT, and subsequent qPCR analysis was consistent with the amplification of PDGFRA. MYCN amplification was seen in 5% of samples being significantly associated with anaplastic astrocytomas (P= 0.03). Overall, DIPG shared similar spectrum of changes to supratentorial HGG with some notable differences, including high-frequency loss of 17p and 14q and lack of CDKN2A/CDKN2B deletion. Informative genetic data providing insight into the underlying biology and potential therapeutic possibilities can be generated from archival tissue and typically small biopsies from DIPG. Our findings highlight the importance of obtaining pretreatment samples.
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