ArticleProteomics. Clinical applications2010
Proteomic analysis of endoscopically (endoscopic pancreatic function test) collected gastroduodenal fluid using in-gel tryptic digestion followed by LC-MS/MS.
Article in Proteomics. Clinical applications, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Post-translational modifications of pancreatic fluid proteins collected via the endoscopic pancreatic function test (ePFT).Journal of proteomics · 2013Trial
- The formation and function of calciprotein particles.Pflugers Archiv : European journal of physiology · 2025Review
- Quantitative proteomic analysis of bronchoalveolar lavage fluid in West Highland white terriers with canine idiopathic pulmonary fibrosis.BMC veterinary research · 2022Article
- Trefoil Factor Family (TFF) Peptides and Their Diverse Molecular Functions in Mucus Barrier Protection and More: Changing the Paradigm.International journal of molecular sciences · 2020Review
- Detection of digestive malignancies and post-gastrectomy complications via gastrointestinal fluid examination.Frontiers of medicine · 2017Review
- Genomics, microRNA, epigenetics, and proteomics for future diagnosis, treatment and monitoring response in upper GI cancers.Clinical and translational medicine · 2016Review
- Inflammatory protein profiling of pancreatic cyst fluid using EUS-FNA in tandem with cytokine microarray differentiates between branch duct IPMN and inflammatory cysts.Journal of immunological methods · 2012Article
- Mass Spectrometry-Based (GeLC-MS/MS) Comparative Proteomic Analysis of Endoscopically (ePFT) Collected Pancreatic and Gastroduodenal Fluids.Clinical and translational gastroenterology · 2012Article
- Proteomic analysis (GeLC-MS/MS) of ePFT-collected pancreatic fluid in chronic pancreatitis.Journal of proteome research · 2012Article
- Mass spectrometry-based proteomics for translational research: a technical overview.The Yale journal of biology and medicine · 2012Review
- Proteomic analysis of formalin-fixed paraffin-embedded pancreatic tissue using liquid chromatography tandem mass spectrometry.Pancreas · 2012Article
- Differentiating Branch Duct and Mixed IPMN in Endoscopically Collected Pancreatic Cyst Fluid via Cytokine Analysis.Gastroenterology research and practice · 2012Article
- Update on endoscopic pancreatic function testing.World journal of gastroenterology · 2011Article
- Difference gel electrophoresis identifies differentially expressed proteins in endoscopically collected pancreatic fluid.Electrophoresis · 2011Article
- Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay.Journal of immunological methods · 2011Article
- Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research.Proteomics. Clinical applications · 2011Review
- Sample preparation for proteomic analysis using a GeLC-MS/MS strategy.Journal of biological methodsArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
purposeProteomic analysis of gastroduodenal fluid offers an alternative strategy to study diseases, such as peptic ulcer disease and gastric cancer. We use in-gel tryptic digestion followed by LC-MS/MS (GeLC-MS/MS) to profile the proteome of gastroduodenal fluid collected during the endoscopic pancreatic function test (ePFT). EXPERIMENTAL
designGastroduodenal fluid specimens collected during ePFT from six patients with upper abdominal pain were subjected to proteomic analysis. We extracted proteins using three chemical precipitation reagents (acetone, ethanol, and trichloroacetic acid) and analyzed each sample by SDS-PAGE and GeLC-MS/MS for protein identification. Cellular origin and molecular function of the identified proteins were determined via gene ontology analysis.
resultsAll three precipitation techniques successfully extracted protein from gastroduodenal fluid, with acetone resulting in excellent resolution and minimal protein degradation compared with the other methods. A total of 134 unique proteins were found in our GeLC-MS/MS analysis of ePFT-collected gastroduodenal fluid samples. Sixty-seven proteins were identified in at least two of the three samples. Gene ontology analysis classified these proteins mainly as being peptidases and localized extracellularly. CONCLUSIONS AND CLINICAL RELEVANCE: ePFT, followed by acetone precipitation, and coupled with LC-MS/MS, can be used to safely collect gastroduodenal fluid from the upper gastrointestinal tract for MS-based proteomic analysis.
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