ArticlePlanta2011
Initiation of somatic embryos and regeneration of plants from primordial shoots of 10-year-old somatic white spruce and expression profiles of 11 genes followed during the tissue culture process.
Article in Planta, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Induction and Marker Selection of Embryogenic-like Callus from the Hypocotyl ofPlants (Basel, Switzerland) · 2026Article
- Woody Plant Transformation: Current Status, Challenges, and Future Perspectives.Plants (Basel, Switzerland) · 2025Review
- Plant genetic transformation: achievements, current status and future prospects.Plant biotechnology journal · 2025Review
- Transcriptome-wide identification and characterization of WUSCHEL-related homeobox (WOX) gene family in Pinus yunnanensis.BMC genomics · 2025Article
- Induction of Somatic Embryogenesis in Plants: Different Players and Focus on WUSCHEL and WUS-RELATED HOMEOBOX (WOX) Transcription Factors.International journal of molecular sciences · 2022Review
- An Improved and Simplified Propagation System for Pollen-Free Sugi (Frontiers in plant science · 2022Article
- Mini review: Application of the somatic embryogenesis technique in conifer species.Forestry research · 2022Review
- Integrated transcriptomic and metabolic analyses provide insights into the maintenance of embryogenic potential and the biosynthesis of phenolic acids and flavonoids involving transcription factors inFrontiers in plant science · 2022Article
- Somatic Embryogenesis in Pines.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Constitutive Overexpression of a ConiferFrontiers in plant science · 2022Article
- Defensive Traits during White Spruce (Insects · 2021Article
- Article
- Peroxidase Enzyme Fractions as Markers of Somatic Embryogenesis Capacities in Olive (Plants (Basel, Switzerland) · 2021Article
- Review
- Progress in Somatic Embryogenesis of Japanese Pines.Frontiers in plant science · 2019Review
- Somatic Embryogenesis Induction in Woody Species: The Future After OMICs Data Assessment.Frontiers in plant science · 2019Review
- Cytological, Biochemical and Molecular Events of the Embryogenic State in Douglas-fir (Frontiers in plant science · 2019Article
- New Approaches to Optimize Somatic Embryogenesis in Maritime Pine.Frontiers in plant science · 2019Article
- Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.].BMC plant biology · 2018Article
- Molecular Dissection of the Regenerative Capacity of Forest Tree Species: Special Focus on Conifers.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adult conifers are notoriously recalcitrant in vegetative propagation and micropropagation that would result in the regeneration of juvenile propagules through somatic embryogenesis (SE) has not been demonstrated to date. Because SE-derived material is more amenable in subsequent tissue culture experiments compared with seed-derived material, a multi-year study was conducted to investigate induction of SE from primordial shoot (PS) explants that were excised from shoot buds of somatic embryo-derived white spruce. The SE induction experiments were carried out first with greenhouse-grown and later with field-grown trees each year from 2002 (2-year-old) to 2010 (10-year-old). Of the four genotypes tested, 893-2 and 893-12 never responded, 893-1 responded up to year 4 and 893-6 consistently responded every year. In 2010, for the first time, three of the 17 893-6 clonal trees produced male strobili as well as SE from cultured PS explants. SE induction was associated with formation of a nodule on the surface of an elongated needle primordium or in callus. Early somatic embryos were detectable after about 3 weeks of culture. Of 11 genes whose expression profiles were followed during the PS cultures, CHAP3A, VP1, WOX2 and SAP2C were expressed exclusively in the early stages of SE, and could potentially be used as markers of embryogenecity. Mature somatic embryos and plants were produced from the explants of responding genotype. Implication of these results for future research on adult conifer recalcitrance in micropropagation is discussed.
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