ArticleMolecular and cellular biology2011
Poly(A) signals located near the 5' end of genes are silenced by a general mechanism that prevents premature 3'-end processing.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Behavioral screening defines the molecular Parkinsonism-related subgroups in Drosophila.Nature communications · 2026Article
- Robust and heritable knockdown of gene expression using a self-cleaving ribozyme in Drosophila.Genetics · 2024Article
- NineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal-ventral patterning.RNA (New York, N.Y.) · 2020Article
- Transcription start site analysis reveals widespread divergent transcription in D. melanogaster and core promoter-encoded enhancer activities.Nucleic acids research · 2018Article
- Article
- Molecular Regulation of Alternative Polyadenylation (APA) within the Drosophila Nervous System.Journal of molecular biology · 2017Article
- Exploring the Impact of Cleavage and Polyadenylation Factors on Pre-mRNA Splicing Across Eukaryotes.G3 (Bethesda, Md.) · 2017Article
- mRNA-Associated Processes and Their Influence on Exon-Intron Structure in Drosophila melanogaster.G3 (Bethesda, Md.) · 2016Article
- Suppressor of sable [Su(s)] and Wdr82 down-regulate RNA from heat-shock-inducible repetitive elements by a mechanism that involves transcription termination.RNA (New York, N.Y.) · 2016Article
- Genome-wide characterization of intergenic polyadenylation sites redefines gene spaces in Arabidopsis thaliana.BMC genomics · 2015Article
- Homozygous Wildtype of XPD K751Q Polymorphism Is Associated with Increased Risk of Nasopharyngeal Carcinoma in Malaysian Population.PloS one · 2015Article
- Transcription: the epicenter of gene expression.Journal of Zhejiang University. Science. B · 2014Article
- RNA polymerase II transcription elongation control.Chemical reviews · 2013Review
- Competition within Introns: Splicing Wins over Polyadenylation via a General Mechanism.Acta naturae · 2013Article
- Alterations in polyadenylation and its implications for endocrine disease.Frontiers in endocrinology · 2013Article
- Promoter-proximal polyadenylation sites reduce transcription activity.Genes & development · 2012Article
- Article
- Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length.The EMBO journal · 2011Article
- Genome-wide landscape of polyadenylation in Arabidopsis provides evidence for extensive alternative polyadenylation.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Unravelling the means to an end: RNA polymerase II transcription termination.Nature reviews. Molecular cell biology · 2011Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
Poly(A) signals located at the 3' end of eukaryotic genes drive cleavage and polyadenylation at the same end of pre-mRNA. Although these sequences are expected only at the 3' end of genes, we found that strong poly(A) signals are also predicted within the 5' untranslated regions (UTRs) of many Drosophila melanogaster mRNAs. Most of these 5' poly(A) signals have little influence on the processing of the endogenous transcripts, but they are very active when placed at the 3' end of reporter genes. In investigating these unexpected observations, we discovered that both these novel poly(A) signals and standard poly(A) signals become functionally silent when they are positioned close to transcription start sites in either Drosophila or human cells. This indicates that the stage when the poly(A) signal emerges from the polymerase II (Pol II) transcription complex determines whether a putative poly(A) signal is recognized as functional. The data suggest that this mechanism, which probably prevents cryptic poly(A) signals from causing premature transcription termination, depends on low Ser2 phosphorylation of the C-terminal domain of Pol II and inefficient recruitment of processing factors.
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