ArticleNucleic acids research2011
A zinc finger protein array for the visual detection of specific DNA sequences for diagnostic applications.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Advances in Double-Stranded DNA Targeting Technologies.Exploration (Beijing, China) · 2026Review
- Signal amplified colorimetric nucleic acid detection based on autocatalytic hairpin assembly.RSC advances · 2024Article
- Application of Engineered Zinc Finger Proteins Immobilized on Paramagnetic Beads for Multiplexed Detection of Pathogenic DNA.Journal of microbiology and biotechnology · 2021Article
- Pathogen-specific DNA sensing with engineered zinc finger proteins immobilized on a polymer chip.The Analyst · 2018Article
- Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research.Molecules and cells · 2017Review
- Exploiting the recognition code for elucidating the mechanism of zinc finger protein-DNA interactions.BMC genomics · 2016Article
- An ensemble micro neural network approach for elucidating interactions between zinc finger proteins and their target DNA.BMC genomics · 2016Article
- Multiplexed detection of pathogen-specific DNA using engineered zinc finger proteins without target amplification.Analytical methods : advancing methods and applications · 2016Article
- Synthetic biology devices for in vitro and in vivo diagnostics.Proceedings of the National Academy of Sciences of the United States of America · 2015Review
- Sensitive and direct electrochemical detection of double-stranded DNA utilizing alkaline phosphatase-labelled zinc finger proteins.The Analyst · 2015Article
- Evaluation of novel design strategies for developing zinc finger nucleases tools for treating human diseases.Biotechnology research international · 2014Article
- Quantitative analysis of TALE-DNA interactions suggests polarity effects.Nucleic acids research · 2013Article
- Highly active zinc-finger nucleases by extended modular assembly.Genome research · 2013Article
- Specific labeling of zinc finger proteins using noncanonical amino acids and copper-free click chemistry.Bioconjugate chemistry · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
The visual detection of specific double-stranded DNA sequences possesses great potential for the development of diagnostics. Zinc finger domains provide a powerful scaffold for creating custom DNA-binding proteins that recognize specific DNA sequences. We previously demonstrated sequence-enabled reassembly of TEM-1 β-lactamase (SEER-LAC), a system consisting of two inactive fragments of β-lactamase each linked to engineered zinc finger proteins (ZFPs). Here the SEER-LAC system was applied to develop ZFP arrays that function as simple devices to identify bacterial double-stranded DNA sequences. The ZFP arrays provided a quantitative assay with a detection limit of 50 fmol of target DNA. The method could distinguish target DNA from non-target DNA within 5 min. The ZFP arrays provided sufficient sensitivity and high specificity to recognize specific DNA sequences. These results suggest that ZFP arrays have the potential to be developed into a simple and rapid point-of-care (POC) diagnostic for the multiplexed detection of pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.