ArticleThe protein journal2011
Characterization of a single-stranded DNA-binding protein from Pseudomonas aeruginosa PAO1.
Article in The protein journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
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- Crystal Structure of an SSB Protein fromInternational journal of molecular sciences · 2022Article
- A Complexed Crystal Structure of a Single-Stranded DNA-Binding Protein with Quercetin and the Structural Basis of Flavonol Inhibition Specificity.International journal of molecular sciences · 2022Article
- Characterization of the Chimeric PriB-SSBc Protein.International journal of molecular sciences · 2021Article
- Characterization of an SSB-dT25 complex: structural insights into the S-shaped ssDNA binding conformation.RSC advances · 2019Article
- The glycine-rich flexible region in SSB is crucial for PriA stimulation.RSC advances · 2018Article
- SAAV2152 is a single-stranded DNA binding protein: the third SSB inOncotarget · 2018Article
- Staphylococcus aureus single-stranded DNA-binding protein SsbA can bind but cannot stimulate PriA helicase.PloS one · 2017Article
- C-terminal domain swapping of SSB changes the size of the ssDNA binding site.BioMed research international · 2014Article
- Yeast two-hybrid analysis of PriB-interacting proteins in replication restart primosome: a proposed PriB-SSB interaction model.The protein journal · 2013Article
- Characterization of flavonol inhibition of DnaB helicase: real-time monitoring, structural modeling, and proposed mechanism.Journal of biomedicine & biotechnology · 2012Article
- Characterization of the single stranded DNA binding protein SsbB encoded in the Gonoccocal Genetic Island.PloS one · 2012Article
- Biochemical characterization of allantoinase from Escherichia coli BL21.The protein journal · 2011Article
- Characterization of a single-stranded DNA binding protein from Salmonella enterica serovar Typhimurium LT2.The protein journal · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-stranded DNA-binding protein (SSB) plays an important role in DNA metabolism, such as in DNA replication, repair, and recombination, and is essential for cell survival. We characterized the single-stranded DNA (ssDNA)-binding properties of Pseudomonas aeruginosa PAO1 SSB (PaSSB) by using fluorescence quenching measurements and electrophoretic mobility shift analysis (EMSA). Analysis of purified PaSSB by gel filtration chromatography revealed a stable tetramer in solution. In fluorescence titrations, PaSSB bound 22-32 nucleotides (nt) per tetramer depending on salt concentration. Using EMSA, we characterized the stoichiometry of PaSSB complexed with a series of ssDNA homopolymers, and the size of the binding site was determined to be 29 ± 1 nt. Furthermore, EMSA results indicated that the dissociation constants of PaSSB for the first tetramer were less than those for the second tetramer. On the basis of these biophysical analyses, the ssDNA binding mode of PaSSB is expected to be noncooperative.
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