ArticleCellular and molecular bioengineering2010
Rho Kinase Regulation of Fibroblast Migratory Mechanics in Fibrillar Collagen Matrices.
Article in Cellular and molecular bioengineering, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Electric field promotes dermal fibroblast transdifferentiation through activation of RhoA/ROCK1 pathway.International journal of medical sciences · 2023Article
- Exogenous extracellular matrix proteins decrease cardiac fibroblast activation in stiffening microenvironment through CAPG.Journal of molecular and cellular cardiology · 2021Article
- Keratocyte mechanobiology.Experimental eye research · 2020Review
- Direct signaling of TL1A-DR3 on fibroblasts induces intestinal fibrosis in vivo.Scientific reports · 2020Article
- In vivo immune cell dynamics in the human cornea.Experimental eye research · 2020Observational
- An In Vitro Model for Assessing Corneal Keratocyte Spreading and Migration on Aligned Fibrillar Collagen.Journal of functional biomaterials · 2018Article
- Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation.Molecular neurobiology · 2018Article
- Fibroblast-fibronectin patterning and network formation in 3D fibrin matrices.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- MCPIP1 Regulates Alveolar Macrophage Apoptosis and Pulmonary Fibroblast Activation After in vitro Exposure to Silica.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Mechanical interactions and crosstalk between corneal keratocytes and the extracellular matrix.Experimental eye research · 2015Review
- The Role of Thrombin and Cell Contractility in Regulating Clustering and Collective Migration of Corneal Fibroblasts in Different ECM Environments.Investigative ophthalmology & visual science · 2015Article
- MMP regulation of corneal keratocyte motility and mechanics in 3-D collagen matrices.Experimental eye research · 2014Article
- Techniques for assessing 3-D cell-matrix mechanical interactions in vitro and in vivo.Experimental cell research · 2013Review
- Purine receptor mediated actin cytoskeleton remodeling of human fibroblasts.Cell calcium · 2013Article
- Corneal stromal cells use both high- and low-contractility migration mechanisms in 3-D collagen matrices.Experimental cell research · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Migration of activated corneal fibroblasts plays an important role in matrix patterning during embryonic development and wound repopulation following injury or refractive surgery. In this study, we investigate the role of Rho kinase in regulating fibroblast migration mechanics, by modifying a previously described nested collagen matrix model to facilitate dynamic imaging of cell-matrix interactions.Human corneal fibroblasts were cultured in nested matrices with media containing either 1% fetal bovine serum (FBS), or 1% FBS plus the Rho kinase inhibitor Y-27632. Time-lapse DIC imaging of cell and extracellular matrix (ECM) movements was performed for up to 72 hours. In addition, static confocal imaging was used to assess 3-D cell morphology and local matrix reorganization.In 1% FBS, significant tractional forces were generated during migration, as indicated by inward displacement and reorganization of collagen in front of cells. When Rho kinase was inhibited, cells became more elongated, and extended dendritic processes into the outer matrix. Interestingly, these dendritic cells were still able to generate tractional forces at their leading edge, whereas cell translocation was substantially reduced. Overall, the data suggests that Rho kinase impacts 3-D fibroblast migration by affecting morphology, polarization, and mechanical coordination between the leading and trailing edges of cells.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.