ArticleOncogene2011
Ultraviolet B irradiation and activation of protein kinase D in primary mouse epidermal keratinocytes.
Article in Oncogene, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- A phosphoproteomic approach reveals that PKD3 controls PKA-mediated glucose and tyrosine metabolism.Life science alliance · 2021Article
- Proanthocyanidins against Oxidative Stress: From Molecular Mechanisms to Clinical Applications.BioMed research international · 2018Review
- Regulation of the Glycerol Transporter, Aquaporin-3, by Histone Deacetylase-3 and p53 in Keratinocytes.The Journal of investigative dermatology · 2017Article
- Protein kinase D signaling in cancer: A friend or foe?Biochimica et biophysica acta. Reviews on cancer · 2017Review
- Mitochondrial and Oxidative Stress-Mediated Activation of Protein Kinase D1 and Its Importance in Pancreatic Cancer.Frontiers in oncology · 2017Review
- Opposing growth regulatory roles of protein kinase D isoforms in human keratinocytes.The Journal of biological chemistry · 2015Article
- Protein kinase D1 deficiency promotes differentiation in epidermal keratinocytes.Journal of dermatological science · 2014Article
- Protein kinase C and Src family kinases mediate angiotensin II-induced protein kinase D activation and acute aldosterone production.Molecular and cellular endocrinology · 2014Article
- A role for phospholipase D in angiotensin II-induced protein kinase D activation in adrenal glomerulosa cell models.Molecular and cellular endocrinology · 2013Article
- Role of VEGF receptors in normal and psoriatic human keratinocytes: evidence from irradiation with different UV sources.PloS one · 2013Article
- PKD2 and PKD3 promote prostate cancer cell invasion by modulating NF-κB- and HDAC1-mediated expression and activation of uPA.Journal of cell science · 2012Article
- Regulation of protein kinase D1 activity.Molecular pharmacology · 2012Review
- Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor.The Journal of biological chemistry · 2011Article
- Emerging roles of protein kinase D1 in cancer.Molecular cancer research : MCR · 2011Review
- Ultraviolet activation of PKD: implications for skin cancer.Future oncology (London, England) · 2011Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Our previous studies demonstrated that protein kinase D (PKD), a serine/threonine kinase implicated in various cell processes, is upregulated in basal cell carcinoma (BCC), supporting a possible tumorigenic role for PKD in skin. As the greatest risk factor for BCC is sun exposure, the ability of ultraviolet B (UVB) irradiation to activate PKD in primary mouse keratinocytes was investigated. Using western analysis with two autophosphorylation-specific antibodies, we show for the first time that UVB activated PKD in a time- and dose-dependent manner. UVB-induced PKD activation was verified using an in vitro kinase assay. Furthermore, activation was reduced by antioxidant pretreatment, suggesting a link with oxidative stress. UVB-induced PKD activation was mediated primarily by Src family tyrosine kinases rather than protein kinase C (PKC), and in fact, UVB did not alter PKC-mediated transphosphorylation. UVB induced apoptosis dose dependently, and this death could be prevented by overexpression of wild-type PKD, but not mutant PKD or the empty adenovirus. Indeed, a mutant that cannot be phosphorylated by Src kinases exacerbated UVB-elicited apoptosis. Thus, our data indicate that UVB irradiation of keratinocytes induces Src-mediated activation of PKD, which protects cells from UVB-stimulated apoptosis, providing a possible explanation for the observed upregulation of PKD in BCC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.