ArticleNucleic acids research2011
Divergent GW182 functional domains in the regulation of translational silencing.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- PABPC1--mRNA stability, protein translation and tumorigenesis.Frontiers in oncology · 2022Pooled it
- Neuron type-specific translatomes in dorsal hippocampus during early memory consolidation.Nature communications · 2026Article
- Article
- Neuron type-specific mRNA translation programs provide a gateway for memory consolidation.bioRxiv : the preprint server for biology · 2025Article
- Identification of pathological pathways centered on circRNA dysregulation in association with irreversible progression of Alzheimer's disease.Genome medicine · 2024Article
- KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182.Nucleic acids research · 2019Article
- Article
- MicroRNAs recruit eIF4E2 to repress translation of target mRNAs.Protein & cell · 2017Article
- The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets.Nucleic acids research · 2013Article
- Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A molecular link between miRISCs and deadenylases provides new insight into the mechanism of gene silencing by microRNAs.Cold Spring Harbor perspectives in biology · 2012Review
- Defining a new role of GW182 in maintaining miRNA stability.EMBO reports · 2012Article
- Mechanism of allele-selective inhibition of huntingtin expression by duplex RNAs that target CAG repeats: function through the RNAi pathway.Nucleic acids research · 2012Article
- MicroRNAs in rheumatoid arthritis.FEBS letters · 2011Review
- PABP is not essential for microRNA-mediated translational repression and deadenylation in vitro.The EMBO journal · 2011Article
- miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs.Nature structural & molecular biology · 2011Article
- MicroRNA in TLR signaling and endotoxin tolerance.Cellular & molecular immunology · 2011Review
- MicroRNAs in systemic rheumatic diseases.Arthritis research & therapy · 2011Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
MicroRNA (miRNA)-mediated gene regulation has become a major focus in many biological processes. GW182 and its long isoform TNGW1 are marker proteins of GW/P bodies and bind to Argonaute proteins of the RNA induced silencing complex. The goal of this study is to further define and distinguish the repression domain(s) in human GW182/TNGW1. Two non-overlapping regions, Δ12 (amino acids 896-1219) containing the Ago hook and Δ5 (amino acids 1670-1962) containing the RRM, both induced comparable silencing in a tethering assay. Mapping data showed that the RRM and its flanking sequences in Δ5, but not the Ago hook in Δ12, were important for silencing. Repression mediated by Δ5 or Δ12 was not differentially affected when known endogenous repressors RCK/p54, GW182/TNGW1, TNRC6B were depleted. Transfected Δ5, but not Δ12, enhanced Ago2-mediated repression in a tethering assay. Transfected Δ12, but not Δ5, released endogenous miRNA reporter silencing without affecting siRNA function. Alanine substitution showed that GW/WG motifs in Δ12 (Δ12a, amino acids 896-1045) were important for silencing activity. Although Δ12 appeared to bind PABPC1 more efficiently than Δ5, neither Δ5 nor Δ12 significantly enhanced reporter mRNA degradation. These different functional characteristics of Δ5 and Δ12 suggest that their roles are distinct, and possibly dynamic, in human GW182-mediated silencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.