ArticleMetabolic engineering2011
Comprehensive engineering of Escherichia coli for enhanced expression of IgG antibodies.
Article in Metabolic engineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Article
- Strategies to Enhance Periplasmic Recombinant Protein Production Yields inFrontiers in bioengineering and biotechnology · 2021Review
- Editorial: Protein Aggregation and Solubility in Microorganisms (Archaea, Bacteria and Unicellular Eukaryotes): Implications and Applications.Frontiers in microbiology · 2020Article
- Solubility, Stability, and Avidity of Recombinant Antibody Fragments Expressed in Microorganisms.Frontiers in microbiology · 2020Review
- Characterization of the internal translation initiation region in monoclonal antibodies expressed inThe Journal of biological chemistry · 2019Article
- Article
- Efficient Antibody Assembly in E. coli Periplasm by Disulfide Bond Folding Factor Co-expression and Culture Optimization.Applied biochemistry and biotechnology · 2017Article
- Isolating Escherichia coli strains for recombinant protein production.Cellular and molecular life sciences : CMLS · 2017Review
- Efficient Production of a Bioactive Bevacizumab Monoclonal Antibody Using the 2A Self-cleavage Peptide in Transgenic Rice Callus.Frontiers in plant science · 2016Article
- Optimizing antibody expression by using the naturally occurring framework diversity in a live bacterial antibody display system.Scientific reports · 2015Article
- Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs.Microbial cell factories · 2015Review
- Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria.Nature communications · 2015Article
- Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies.Microbial cell factories · 2014Article
- Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii.AMB Express · 2014Article
- Unnatural amino acid incorporation in E. coli: current and future applications in the design of therapeutic proteins.Frontiers in chemistry · 2014Review
- Recombinant polypeptide production in E. coli: towards a rational approach to improve the yields of functional proteins.Microbial cell factories · 2013Review
- Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies.Nature biotechnology · 2013Article
- Multi-copy genes that enhance the yield of mammalian G protein-coupled receptors in Escherichia coli.Metabolic engineering · 2012Article
- Combinatorial approaches for inverse metabolic engineering applications.Computational and structural biotechnology journal · 2012Review
- Discovery of a new class of inhibitors for the protein arginine deiminase type 4 (PAD4) by structure-based virtual screening.BMC bioinformatics · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The expression of IgG antibodies in Escherichia coli is of increasing interest for analytical and therapeutic applications. In this work, we describe a comprehensive and systematic approach to the development of a dicistronic expression system for enhanced IgG expression in E. coli encompassing: (i) random mutagenesis and high-throughput screening for the isolation of over-expressing strains using flow cytometry and (ii) optimization of translation initiation via the screening of libraries of synonymous codons in the 5' region of the second cistron (heavy chain). The effects of different promoters and co-expression of molecular chaperones on full-length IgG production were also investigated. The optimized system resulted in reliable expression of fully assembled IgG at yields between 1 and 4 mg/L of shake flask culture for different antibodies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.