ArticleBiochemical and biophysical research communications2011
Sensitive and quantitative detection of botulinum neurotoxin in neurons derived from mouse embryonic stem cells.
Article in Biochemical and biophysical research communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Recent Developments in Botulinum Neurotoxins Detection.Microorganisms · 2022Review
- Critical Analysis of Neuronal Cell and the Mouse Bioassay for Detection of Botulinum Neurotoxins.Toxins · 2019Review
- Embryonic Stem Cell-Derived Neurons Grown on Multi-Electrode Arrays as a NovelFrontiers in pharmacology · 2017Article
- Article
- Development of a Highly Sensitive Cell-Based Assay for Detecting Botulinum Neurotoxin Type A through Neural Culture Media Optimization.Journal of biomolecular screening · 2016Article
- Functional evaluation of biological neurotoxins in networked cultures of stem cell-derived central nervous system neurons.Journal of visualized experiments : JoVE · 2015Article
- Article
- Recent developments in cell-based assays and stem cell technologies for botulinum neurotoxin research and drug discovery.Expert review of molecular diagnostics · 2014Review
- Characterization of botulinum neurotoxin A subtypes 1 through 5 by investigation of activities in mice, in neuronal cell cultures, and in vitro.Infection and immunity · 2013Article
- Progress in cell based assays for botulinum neurotoxin detection.Current topics in microbiology and immunology · 2013Review
- Model for studying Clostridium botulinum neurotoxin using differentiated motor neuron-like NG108-15 cells.Biochemical and biophysical research communications · 2012Article
- Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection.Toxicological sciences : an official journal of the Society of Toxicology · 2012Article
- Botulinum neurotoxin serotype A specific cell-based potency assay to replace the mouse bioassay.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Botulinum neurotoxins (BoNTs), the most poisonous protein toxins known, represent a serious bioterrorism threat but are also used as a unique and important bio-pharmaceutical to treat an increasing myriad of neurological disorders. The only currently accepted detection method by the United States Food and Drug Administration for biological activity of BoNTs and for potency determination of pharmaceutical preparations is the mouse bioassay (MBA). Recent advances have indicated that cell-based assays using primary neuronal cells can provide an equally sensitive and robust detection platform as the MBA to reliably and quantitatively detect biologically active BoNTs. This study reports for the first time a BoNT detection assay using mouse embryonic stem cells to produce a neuronal cell culture. The data presented indicate that this assay can reliably detect BoNT/A with a similar sensitivity as the MBA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.