ArticleNucleosides, nucleotides & nucleic acids2010
Labeled nucleoside triphosphates with reversibly terminating aminoalkoxyl groups.
Article in Nucleosides, nucleotides & nucleic acids, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Semi-rational engineering of terminal deoxynucleotidyl transferase for high-efficiency enzymaticSynthetic and systems biotechnology · 2026Article
- Engineering a Virus-Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Analysis and control of untemplated DNA polymerase activity for guided synthesis of kilobase-scale DNA sequences.Nature communications · 2026Article
- Evolving a terminal deoxynucleotidyl transferase for commercial enzymatic DNA synthesis.Nucleic acids research · 2025Article
- Controlled enzymatic synthesis of oligonucleotides.Communications chemistry · 2024Review
- Multiplex enzymatic synthesis of DNA with single-base resolution.Science advances · 2023Article
- Spatially Selective Electrochemical Cleavage of a Polymerase-Nucleotide Conjugate.ACS synthetic biology · 2023Article
- DNA synthesis technologies to close the gene writing gap.Nature reviews. Chemistry · 2023Review
- Towards the controlled enzymatic synthesis of LNA containing oligonucleotides.Frontiers in chemistry · 2023Article
- CMOS electrochemical pH localizer-imager.Science advances · 2022Article
- Evaluation of 3'-phosphate as a transient protecting group for controlled enzymatic synthesis of DNA and XNA oligonucleotides.Communications chemistry · 2022Article
- Modified nucleoside triphosphates in bacterial research forRSC chemical biology · 2020Article
- Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates.Frontiers in molecular biosciences · 2019Review
- Unlocking P(V): Reagents for chiral phosphorothioate synthesis.Science (New York, N.Y.) · 2018Article
- De novo DNA synthesis using polymerase-nucleotide conjugates.Nature biotechnology · 2018Article
- Template-Independent Enzymatic Oligonucleotide Synthesis (TiEOS): Its History, Prospects, and Challenges.Biochemistry · 2018Review
- Synthesis of O-Amino Sugars and Nucleosides.Molecules (Basel, Switzerland) · 2018Review
- DNA polymerases drive DNA sequencing-by-synthesis technologies: both past and present.Frontiers in microbiology · 2014Review
- The history and advances of reversible terminators used in new generations of sequencing technology.Genomics, proteomics & bioinformatics · 2013Review
- Rapid incorporation kinetics and improved fidelity of a novel class of 3'-OH unblocked reversible terminators.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Nucleoside triphosphates having a 3'-ONH₂ blocking group have been prepared with and without fluorescent tags on their nucleobases. DNA polymerases were identified that accepted these, adding a single nucleotide to the 3'-end of a primer in a template-directed extension reaction that then stops. Nitrite chemistry was developed to cleave the 3'-ONH₂ group under mild conditions to allow continued primer extension. Extension-cleavage-extension cycles in solution were demonstrated with untagged nucleotides and mixtures of tagged and untagged nucleotides. Multiple extension-cleavage-extension cycles were demonstrated on an Intelligent Bio-Systems Sequencer, showing the potential of the 3'-ONH₂ blocking group in "next generation sequencing."
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.