ArticleCurrent microbiology2011
Reverse transcription loop-mediated isothermal amplification for rapid detection of transmissible gastroenteritis virus.
Article in Current microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- A crRNA/Cas12a complex-driven rapid and visual detection method for four porcine diarrhea viruses.BMC veterinary research · 2026Article
- Rapid and visual detection of transmissible gastroenteritis virus using a CRISPR/Cas12a system combined with loop-mediated isothermal amplification.BMC veterinary research · 2025Article
- Development of a CRISPR-Cas12a based assay for the detection of swine enteric coronaviruses in pig herds in China.Advanced biotechnology · 2024Article
- Porcine enteric coronaviruses: an updated overview of the pathogenesis, prevalence, and diagnosis.Veterinary research communications · 2021Review
- Article
- Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model.The Journal of veterinary medical science · 2019Article
- Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification.Journal of virological methods · 2018Article
- Rapid detection of equine coronavirus by reverse transcription loop-mediated isothermal amplification.Journal of virological methods · 2015Article
- Ultrasensitive Detection of RNA and DNA Viruses Simultaneously Using Duplex UNDP-PCR Assay.PloS one · 2015Article
- A phage-displayed peptide recognizing porcine aminopeptidase N is a potent small molecule inhibitor of PEDV entry.Virology · 2014Article
- Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses.Journal of virological methods · 2013Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transmissible gastroenteritis virus (TGEV) is the causative agent of porcine transmissible gastroenteritis, and sensitive detection methods are required for preventing the disease. In this article, reverse transcription-loop-mediated isothermal amplification (RT-LAMP) was developed to detect TGEV. Three pairs of primers targeting the nucleocapsid (N) gene of TGEV were synthesized and used in the RT-LAMP. The optimization, sensitivity, and specificity of the RT-LAMP were evaluated. Our results showed that the RT-LAMP amplified the N gene with high specificity, efficiency, and rapidity at isothermal condition. The optimal reaction condition was achieved at 60°C for 30 min. The RT-LAMP assay was more sensitive than gel-based RT-PCR and PCR. It had a higher sensitivity than enzyme-linked immunosorbent assay (ELISA) using the equal virus templates. In addition, the established RT-LAMP differentiated TGEV from porcine epidemic diarrhea virus, porcine rotavirus, porcine pseudorabies virus, porcine reproductive and respiratory syndrome virus, and avian infectious bronchitis virus. The approach is suitable for detecting TGEV for field diagnostics or in less-equipped laboratories due to its convenience and simplicity.
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