ArticleNature protocols2010
Disaccharide compositional analysis of heparan sulfate and heparin polysaccharides using UV or high-sensitivity fluorescence (BODIPY) detection.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- A novel inhibitor of SARS-CoV infection: Lactulose octasulfate interferes with ACE2-Spike protein binding.Heliyon · 2024Article
- Analysis of Heparin Samples by Attenuated Total Reflectance Fourier-Transform Infrared Spectroscopy in the Solid State.ACS central science · 2023Article
- Improving the Sensitivity for Quantifying Heparan Sulfate from Biological Samples.Analytical chemistry · 2021Article
- Chemical O-sulfation of N-sulfoheparosan: a route to rare N-sulfo-3-O-sulfoglucosamine and 2-O-sulfoglucuronic acid.Glycoconjugate journal · 2020Article
- Tools for the Quality Control of Pharmaceutical Heparin.Medicina (Kaunas, Lithuania) · 2019Review
- A mutant-cell library for systematic analysis of heparan sulfate structure-function relationships.Nature methods · 2018Article
- Surveying silicon nitride nanopores for glycomics and heparin quality assurance.Nature communications · 2018Article
- LC-MS/MS characterization of xyloside-primed glycosaminoglycans with cytotoxic properties reveals structural diversity and novel glycan modifications.The Journal of biological chemistry · 2018Article
- Glycosaminoglycanomics: where we are.Glycoconjugate journal · 2017Review
- Heparan Sulfate Identification and Characterisation: Method II. Enzymatic Depolymerisation and SAX-HPLC Analysis to Determine Disaccharide Composition.Bio-protocol · 2017Article
- Site-specific identification of heparan and chondroitin sulfate glycosaminoglycans in hybrid proteoglycans.Scientific reports · 2016Article
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- Article
- Capillary electrophoresis for total glycosaminoglycan analysis.Analytical and bioanalytical chemistry · 2014Article
- Analysis of glycosaminoglycan-derived, precolumn, 2-aminoacridone-labeled disaccharides with LC-fluorescence and LC-MS detection.Nature protocols · 2014Article
- Recent advances in cellular glycomic analyses.Biomolecules · 2013Article
- Article
- Glycomics approaches for the bioassay and structural analysis of heparin/heparan sulphates.Metabolites · 2012Article
- Signal amplification by glyco-qPCR for ultrasensitive detection of carbohydrates: applications in glycobiology.Angewandte Chemie (International ed. in English) · 2012Article
- Differential sulfation remodelling of heparan sulfate by extracellular 6-O-sulfatases regulates fibroblast growth factor-induced boundary formation by glial cells: implications for glial cell transplantation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
One of the first steps in characterizing heparan sulfate (HS) and its close relative heparin is to conduct disaccharide composition analysis. This provides an overall picture of the structure of the polysaccharide in terms of its constituent disaccharides. This is of importance, for example, in the initial characterization of spatially and temporally regulated structures. Two protocols for conducting disaccharide analysis are presented here, both exploiting exhaustive digestion of the polysaccharide, yielding constituent disaccharides, by bacterial heparin lyases. The first method, suitable for microgram quantities of material, relies on the separation of the disaccharides by high-performance liquid chromatography (HPLC) coupled to ultraviolet absorbance detection and can be performed in 2 d. The second exploits reducing end-labeling with the fluorophore BODIPY hydrazide, separation by HPLC, and subsequent fluorescence detection and quantitation. The latter is a high-sensitivity method that requires nanograms of starting material and has a detection limit in the low fmol range, and is thus the most sensitive method for disaccharide compositional analysis of HS yet reported. Fluorescence detection can be routinely carried out in 3 d.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.