ArticleThe Journal of biological chemistry2011
TRPC5 channel sensitivities to antioxidants and hydroxylated stilbenes.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Vitamin C Modes of Action in Calcium-Involved Signaling in the Brain.Antioxidants (Basel, Switzerland) · 2023Review
- Review
- Reactive Oxygen Species Cause Exercise-Induced Angina in a Myocardial Ischaemia-Reperfusion Injury Model.International journal of molecular sciences · 2022Article
- TRP channels in COVID-19 disease: Potential targets for prevention and treatment.Chemico-biological interactions · 2021Review
- Evidence of a Role for the TRPC Subfamily in Mediating Oxidative Stress in Parkinson's Disease.Frontiers in physiology · 2020Review
- Isoliquiritigenin Inhibits Atherosclerosis by Blocking TRPC5 Channel Expression.Cardiovascular therapeutics · 2020Article
- Antinociception by the anti-oxidized phospholipid antibody E06.British journal of pharmacology · 2018Article
- Transient Receptor Potential Canonical Channels 4 and 5 MediateOxidative medicine and cellular longevity · 2018Article
- Integration of TRPC6 and NADPH oxidase activation in lysophosphatidylcholine-induced TRPC5 externalization.American journal of physiology. Cell physiology · 2017Article
- Natural and synthetic flavonoid modulation of TRPC5 channels.British journal of pharmacology · 2016Article
- Hypercholesterolemia inhibits re-endothelialization of arterial injuries by TRPC channel activation.Journal of vascular surgery · 2015Article
- Gallic acid functions as a TRPA1 antagonist with relevant antinociceptive and antiedematogenic effects in mice.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
- Resveratrol and calcium signaling: molecular mechanisms and clinical relevance.Molecules (Basel, Switzerland) · 2014Review
- In pursuit of small molecule chemistry for calcium-permeable non-selective TRPC channels -- mirage or pot of gold?British journal of pharmacology · 2013Review
- Inhibition of endothelial cell Ca²⁺ entry and transient receptor potential channels by Sigma-1 receptor ligands.British journal of pharmacology · 2013Article
- Constitutively active TRPC channels of adipocytes confer a mechanism for sensing dietary fatty acids and regulating adiponectin.Circulation research · 2012Article
- Orai1 calcium channels in the vasculature.Pflugers Archiv : European journal of physiology · 2012Review
- Integration of transient receptor potential canonical channels with lipids.Acta physiologica (Oxford, England) · 2012Review
- GVI phospholipase A2 role in the stimulatory effect of sphingosine-1-phosphate on TRPC5 cationic channels.Cell calcium · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Transient receptor potential canonical 5 (TRPC5) forms cationic channels that are polymodal sensors of factors including oxidized phospholipids, hydrogen peroxide, and reduced thioredoxin. The aim of this study was to expand knowledge of the chemical-sensing capabilities of TRPC5 by investigating dietary antioxidants. Human TRPC5 channels were expressed in HEK 293 cells and studied by patch clamp and intracellular Ca(2+) recording. GFP- and HA-tagged channels were used to quantify plasma membrane localization. Gallic acid and vitamin C suppressed TRPC5 activity if it was evoked by exogenous hydrogen peroxide or lanthanide ions but not by lysophosphatidylcholine or carbachol. Catalase mimicked the effects, suggesting that lanthanide-evoked activity depended on endogenous hydrogen peroxide. Trans-resveratrol, by contrast, inhibited all modes of TRPC5, and its effect was additive with that of vitamin C, suggesting antioxidant-independent action. The IC(50) was ∼10 μM. Diethylstilbestrol, a related hydroxylated stilbene, inhibited TRPC5 with a similar IC(50), but its action contrasted sharply with that of resveratrol in outside-out membrane patches where diethylstilbestrol caused strong and reversible inhibition and resveratrol had no effect, suggesting indirect modulation by resveratrol. Resveratrol did not affect channel surface density, but its effect was calcium-sensitive, indicating an action via a calcium-dependent intermediate. The data suggest previously unrecognized chemical-sensing properties of TRPC5 through multiple mechanisms: (i) inhibition by scavengers of reactive oxygen species because a mode of TRPC5 activity depends on endogenous hydrogen peroxide; (ii) direct channel blockade by diethylstilbestrol; and (iii) indirect, antioxidant-independent inhibition by resveratrol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.