ArticleThe Journal of biological chemistry2011
Identification and characterization of endogenous galectins expressed in Madin Darby canine kidney cells.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 45 citations in OpenAlex.
- Influence of glycoprotein MUC1 on trafficking of the CaThe Journal of biological chemistry · 2023Article
- Evaluation of the Bactericidal Activity of Galectins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Examination of Whole-Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Examining Galectin Binding Specificity Using Glycan Microarrays.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Evaluation of Galectin Binding by Surface Plasmon Resonance.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cloning, Expression, and Purification of Galectins for In Vitro Studies.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Purification of Recombinant Galectins from Different Species Using Distinct Affinity Chromatography Methods.Methods in molecular biology (Clifton, N.J.) · 2022Article
- First Evidence for a Role of Siglec-8 in Breast Cancer.International journal of molecular sciences · 2021Article
- Identification of targets of IL-13 and STAT6 signaling in polycystic kidney disease.American journal of physiology. Renal physiology · 2018Article
- Novel roles for mucin 1 in the kidney.Current opinion in nephrology and hypertension · 2017Review
- Maltodextrin modified liposomes for drug delivery through the blood-brain barrier.MedChemComm · 2017Article
- Mucin-1 Increases Renal TRPV5 Activity In Vitro, and Urinary Level Associates with Calcium Nephrolithiasis in Patients.Journal of the American Society of Nephrology : JASN · 2016Article
- Thermodynamic Switch in Binding of Adhesion/Growth Regulatory Human Galectin-3 to Tumor-Associated TF Antigen (CD176) and MUC1 Glycopeptides.Biochemistry · 2015Article
- Examination of galectin localization using confocal microscopy.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Evaluation of the bactericidal activity of galectins.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Examination of whole cell galectin binding by solid phase and flow cytometric analysis.Methods in molecular biology (Clifton, N.J.) · 2015Article
- RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.Frontiers in physiology · 2015Article
- Alkylation of galectin-1 with iodoacetamide and mass spectrometric mapping of the sites of incorporation.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Evolving mechanistic insights into galectin functions.Methods in molecular biology (Clifton, N.J.) · 2015Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Madin Darby canine kidney (MDCK) cells are a well characterized epithelial cell line used to study mechanisms of polarized delivery. As glycans on apically expressed proteins have been identified as targeting signals, and crosslinking by the abundant galectin-3 has been implicated in the mechanism of glycan-dependent sorting, we wanted to identify other members of the galectin (Gal) family expressed in MDCK cells. By analyzing intron-exon boundaries, we identified canine genes that were highly homologous to mammalian Gal-1, 2, 3, 4, 7, 8, 9, and 12, and galectin-related HSPC159 and GRIFIN. Transcripts for Gal-2 and -12 were not detected in MDCK cells, but we found transcript levels for Gal-3 > Gal-9 > Gal-8 > Gal-1 ⋙ Gal-4 > Gal-7. Canine Gal-1, -2, -3, -4, -7, -8, -9, and -12 were cloned and expressed in Escherichia coli as GST fusion proteins to characterize binding specificities on arrays of synthetic glycans on glass slides from Core H of the NIH Consortium for Functional Glycomics. Individual expression of the N-terminal (GST-Gal-9N) and C-terminal (GST-Gal-9C) carbohydrate recognition domains greatly improved protein yield and the ability to characterize Gal-9 binding on the array. Canine galectins differentially bound sulfated disaccharides as well as human blood groups A, B, and H on both N-glycans and linear glycan structures on the array. Analysis of GST-Gal-1, -3, -4, -7, -8, -9N, and -9C binding to immunopurified human MUC1 expressed in MDCK cells revealed a preference for binding GST-Gal-3 and -9, which interestingly reflects the two most abundant galectins expressed in MDCK cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.