ArticleInternational journal of pediatric otorhinolaryngology2011
Identification of Streptococcus pneumoniae and Haemophilus influenzae in culture-negative middle ear fluids from children with acute otitis media by combination of multiplex PCR and multi-locus sequencing typing.
Article in International journal of pediatric otorhinolaryngology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Biofilm-Forming Bacteria Implicated in Complex Otitis Media in Children in the Post-Heptavalent Pneumococcal Conjugate Vaccine (PCV7) Era.Microorganisms · 2023Article
- Diagnostic detection of intended bacteria associated with respiratory tract infections among Kelantanese Malaysian Hajj pilgrims by a ready-to-use, thermostable multiplex PCR assay.Saudi journal of biological sciences · 2022Article
- Differentiation of otitis media-causing bacteria and biofilmsFrontiers in cellular and infection microbiology · 2022Article
- Impact of Laboratory Methods and Gene Targets on Detection ofReports of biochemistry & molecular biology · 2020Article
- Article
- Pneumococcal Phenotype and Interaction with Nontypeable Haemophilus influenzae as Determinants of Otitis Media Progression.Infection and immunity · 2018Article
- Three Innate Cytokine Biomarkers Predict Presence of Acute Otitis Media and Relevant Otopathogens.Biomarkers and applications · 2018Article
- Inflammation-associated cytokine analysis identifies presence of respiratory bacterial pathogens in the nasopharynx.Pathogens and disease · 2016Article
- Correlation of nasopharyngeal cultures prior to and at onset of acute otitis media with middle ear fluid cultures.BMC infectious diseases · 2014Article
- Effectiveness of the standard and an alternative set of Streptococcus pneumoniae multi locus sequence typing primers.BMC microbiology · 2014Article
- Classification, identification, and clinical significance of Haemophilus and Aggregatibacter species with host specificity for humans.Clinical microbiology reviews · 2014Review
- Beta- lactam antibiotics stimulate biofilm formation in non-typeable haemophilus influenzae by up-regulating carbohydrate metabolism.PloS one · 2014Article
- Decreased Expression of TLR-9 and Cytokines in the Presence of Bacteria in Patients with Otitis Media with Effusion.Clinical and experimental otorhinolaryngology · 2013Article
- Relationship with original pathogen in recurrence of acute otitis media after completion of amoxicillin/clavulanate: bacterial relapse or new pathogen.The Pediatric infectious disease journal · 2013Article
- Cellular immune response in young children accounts for recurrent acute otitis media.Current allergy and asthma reports · 2013Review
- Characterization of recombinant fluoroquinolone-resistant pneumococcus-like isolates.Antimicrobial agents and chemotherapy · 2013Article
- One third of middle ear effusions from children undergoing tympanostomy tube placement had multiple bacterial pathogens.BMC pediatrics · 2012Article
- Clinical significance of serum S100A12 in acute otitis media in young children.The Pediatric infectious disease journal · 2012Article
- Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveStreptococcus pneumoniae (Spn) and Haemophilus influenzae (Hflu) are major etiologic pathogens for acute otitis media (AOM). However, when Spn and Hflu strains are not identified by traditional culture methods, use of alternative PCR-based diagnosis becomes critical. This study aimed to develop a combined molecular method to accurately detect these otopathogens.
methodsMiddle ear fluid (MEF) samples were collected by tympanocentesis from children with AOM to isolate Spn and Hflu by standard culture procedures. Multiplex PCR (mPCR) and multi-locus sequence typing (MLST) techniques were used to detect Spn and Hflu in culture-negative MEF samples.
resultsWe found 20 Spn or Hflu culture-positive MEF samples that were mPCR-positive and typeable by MLST. The sequences of the housekeeping genes and the MLST allelic profiles obtained from Spn or Hflu culture isolates matched exactly MEF samples that were tested directly without culture isolation. Of 63 MEF samples that were culture-negative for Spn, 38% (24/63) were mPCR-positive for Spn. Of 50 MEF samples that were culture-negative for Hflu, 24% (12/50) were mPCR-positive for Hflu. Among these culture-negative but mPCR-positive MEF samples, 25% (6/24) and 25% (3/12) were typeable by MLST for Spn and Hflu, respectively.
conclusionsMEF samples may be analyzed with mPCR and MLST directly without culture isolation and the addition of mPCR and MLST may accurately identify Spn and Hflu in MEF of children with AOM when bacterial culture is negative.
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