ArticlePloS one2010
Effects of the TLR2 agonists MALP-2 and Pam3Cys in isolated mouse lungs.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 36 citations in OpenAlex.
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- Lipopeptides for Vaccine Development.Bioconjugate chemistry · 2021Review
- Chemokines and chemokine receptors during COVID-19 infection.Computational and structural biotechnology journal · 2021Review
- The Lipocalin2 Gene is Regulated in Mammary Epithelial Cells by NFκB and C/EBP In Response to Mycoplasma.Scientific reports · 2020Article
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- Penehyclidine hydrochloride inhibits TLR4 signaling and inflammation, and attenuates blunt chest trauma and hemorrhagic shock-induced acute lung injury in rats.Molecular medicine reports · 2018Article
- The effects of hydroxyethyl starch and gelatine on pulmonary cytokine production and oedema formation.Scientific reports · 2018Article
- Staphylococcal LTA antagonizes the B cell-mitogenic potential of LPS.Scientific reports · 2018Article
- The IRAK-ERK-p67phox-Nox-2 axis mediates TLR4, 2-induced ROS production for IL-1β transcription and processing in monocytes.Cellular & molecular immunology · 2016Article
- Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino mice.Scientific reports · 2016Article
- Sepsis: in search of cure.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2016Review
- The role of innate immune signaling in the pathogenesis of atopic dermatitis and consequences for treatments.Seminars in immunopathology · 2016Review
- Corilagin Protects Against HSV1 Encephalitis Through Inhibiting the TLR2 Signaling Pathways In Vivo and In Vitro.Molecular neurobiology · 2015Article
- Pyrogen detection methods: comparison of bovine whole blood assay (bWBA) and monocyte activation test (MAT).BMC pharmacology & toxicology · 2014Article
- Animal models of ex vivo lung perfusion as a platform for transplantation research.World journal of experimental medicine · 2014Review
- Fate of TLR-1/TLR-2 agonist functionalised pDNA nanoparticles upon deposition at the human bronchial epithelium in vitro.Journal of nanobiotechnology · 2013Article
- TLR4 as receptor for HMGB1-mediated acute lung injury after liver ischemia/reperfusion injury.Laboratory investigation; a journal of technical methods and pathology · 2013Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGram-positive and Gram-negative bacteria are main causes of pneumonia or acute lung injury. They are recognized by the innate immune system via toll-like receptor-2 (TLR2) or TLR4, respectively. Among all organs, the lungs have the highest expression of TLR2 receptors, but little is known about the pulmonary consequences of their activation. Here we studied the effects of the TLR2/6 agonist MALP-2, the TLR2/1 agonist Pam(3)Cys and the TLR4 agonist lipopolysaccharide (LPS) on pro-inflammatory responses in isolated lungs. METHODOLOGY/PRINCIPAL
findingsIsolated perfused mouse lungs were perfused for 60 min or 180 min with MALP-2 (25 ng/mL), Pam(3)Cys (160 ng/mL) or LPS (1 µg/mL). We studied mediator release by enzyme linked immunosorbent assay (ELISA), the activation of mitogen activated protein kinase (MAPK) and AKT/protein kinase B by immunoblotting, and gene induction by quantitative polymerase chain reaction. All agonists activated the MAPK ERK1/2 and p38, but neither JNK or AKT kinase. The TLR ligands upregulated the inflammation related genes Tnf, Il1β, Il6, Il10, Il12, Ifng, Cxcl2 (MIP-2α) and Ptgs2. MALP-2 was more potent than Pam(3)Cys in inducing Slpi, Cxcl10 (IP10) and Parg. Remarkable was the strong induction of Tnc by MALP2, which was not seen with Pam(3)Cys or LPS. The growth factor related genes Areg and Hbegf were not affected. In addition, all three TLR agonists stimulated the release of IL-6, TNF, CXCL2 and CXCL10 protein from the lungs. CONCLUSIONS/SIGNIFICANCE: TLR2 and TLR4 activation leads to similar reactions in the lungs regarding MAPK activation, gene induction and mediator release. Several genes studied here have not yet been appreciated as targets of TLR2-activation in the lungs before, i.e., Slpi, tenascin C, Parg and Traf1. In addition, the MALP-2 dependent induction of Tnc may indicate the existence of TLR2/6-specific pathways.
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