ArticlePLoS computational biology2010
How cations can assist DNase I in DNA binding and hydrolysis.
Article in PLoS computational biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 87 citations in OpenAlex.
- Chemical modifications to enhance the stability of DNA nanoparticles for biological applications.iScience · 2026Review
- Zerumbone Combined with Hydrolytic Enzymes: A Promising Strategy to Control Mixed Biofilms ofACS omega · 2026Article
- Developmental Progress and Future Potential for Inhaled Biologics in the Treatment of Respiratory Diseases.Drugs · 2026Review
- High-throughput monitoring of free MgAnalytical and bioanalytical chemistry · 2026Article
- Methylene insertion reveals the importance of phosphate-phosphate distance in DNase I cleavage.RSC chemical biology · 2026Article
- DNase I as a probe for unpolymerized actin: revisiting a classic tool for nuclear actin research.Pflugers Archiv : European journal of physiology · 2026Review
- Development and optimization of host DNA depletion in blood cultures using a saponin and salt-activated nuclease-based method.Frontiers in microbiology · 2026Article
- DNA Nanostructure Self-Assembly in an Aqueous Ionic Liquid Solution with Enhanced Stability and Target Binding Affinity.Journal of the American Chemical Society · 2025Article
- A temperature-driven DNA discrimination strategy to distinguish E. coli DNA and phage 5hmC-modified DNA.Nucleic acids research · 2025Article
- Core-Shell ZnOACS applied materials & interfaces · 2025Article
- Development of DNase-1 Loaded Polymeric Nanoparticles Synthesized by Inverse Flash Nanoprecipitation for Neutrophil-Mediated Drug Delivery to In Vitro Thrombi.Advanced healthcare materials · 2025Article
- Tailoring DNA Origami Protection: A Study of Oligolysine-PEG Coatings for Improved Colloidal, Structural, and Functional Integrity.ACS polymers Au · 2025Article
- Different results despite high homology: Comparative expression of human and murine DNase1 in Pichia pastoris.PloS one · 2025Article
- Perish the thawed? EDTA reduces DNA degradation during extraction from frozen tissue.PloS one · 2025Article
- Bile-induced biofilm formation inmBio · 2024Article
- An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig sperm.Biological research · 2023Article
- Review
- Self-Assembly of DNA Nanostructures in Different Cations.Small (Weinheim an der Bergstrasse, Germany) · 2023Article
- Self-assembly of DNA nanostructures in different cations.bioRxiv : the preprint server for biology · 2023Article
- Synthesis and DNase I Inhibitory Properties of New Squaramides.Molecules (Basel, Switzerland) · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNase I requires Ca²+ and Mg²+ for hydrolyzing double-stranded DNA. However, the number and the location of DNase I ion-binding sites remain unclear, as well as the role of these counter-ions. Using molecular dynamics simulations, we show that bovine pancreatic (bp) DNase I contains four ion-binding pockets. Two of them strongly bind Ca²+ while the other two sites coordinate Mg²+. These theoretical results are strongly supported by revisiting crystallographic structures that contain bpDNase I. One Ca²+ stabilizes the functional DNase I structure. The presence of Mg²+ in close vicinity to the catalytic pocket of bpDNase I reinforces the idea of a cation-assisted hydrolytic mechanism. Importantly, Poisson-Boltzmann-type electrostatic potential calculations demonstrate that the divalent cations collectively control the electrostatic fit between bpDNase I and DNA. These results improve our understanding of the essential role of cations in the biological function of bpDNase I. The high degree of conservation of the amino acids involved in the identified cation-binding sites across DNase I and DNase I-like proteins from various species suggests that our findings generally apply to all DNase I-DNA interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.