ArticlePloS one2010
Multi-locus sequence typing of enteroaggregative Escherichia coli isolates from Nigerian children uncovers multiple lineages.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 98 citations in OpenAlex.
- Multidrug-Resistant Pathogen Clonal Spread in Nigeria: A Systematic Review and Meta-Analysis.Health science reports · 2026Review
- EnteroaggregativeEcoSal Plus · 2025Review
- Uropathogenic Escherichia coli (UPEC) that hides its identity: features of LC2 and EC73 strains from recurrent urinary tract infections.BMC microbiology · 2025Article
- Genomic features of three major diarrhoeagenicMicrobial genomics · 2025Article
- Molecular Characterization of Extended-Spectrum ß-Lactamases-ProducingAntibiotics (Basel, Switzerland) · 2025Article
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- Characterization of non-O157 enterohemorrhagic Escherichia coli isolated from different sources in Egypt.BMC microbiology · 2024Article
- DiarrheagenicInternational journal of molecular sciences · 2024Article
- Genomic Dissection of an EnteroaggregativeInternational journal of molecular sciences · 2024Article
- Distribution of Extended-Spectrum-β-Lactamase-Producing DiarrheagenicAntibiotics (Basel, Switzerland) · 2023Article
- Molecular characterization of extraintestinal and diarrheagenicVirulence · 2022Article
- Sporadic Occurrence of Enteroaggregative Shiga Toxin-Producing Escherichia coli O104:H4 Similar to 2011 Outbreak Strain.Emerging infectious diseases · 2022Article
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- Molecular epidemiology of extended-spectrum beta-lactamase-producing extra-intestinal pathogenic Escherichia coli strains over a 2-year period (2017-2019) from Zimbabwe.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Tracking Fecal Bacterial Dispersion from Municipal Wastewater to Peri-Urban Farms during Monsoon Rains in Hue City, Vietnam.International journal of environmental research and public health · 2021Article
- Prevalence, phylogeny, and antimicrobial resistance of Escherichia coli pathotypes isolated from children less than 5 years old with community acquired- diarrhea in Upper Egypt.BMC infectious diseases · 2020Article
- Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India.PLoS neglected tropical diseases · 2020Article
- Redefining enteroaggregative Escherichia coli (EAEC): Genomic characterization of epidemiological EAEC strains.PLoS neglected tropical diseases · 2020Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundEnteroaggregative Escherichia coli (EAEC) are defined by their stacked-brick adherence pattern to human epithelial cells. There is no all-encompassing genetic marker for EAEC. The category is commonly implicated in diarrhea but research is hampered by perplexing heterogeneity. METHODOLOGY/PRINCIPAL
findingsTo identify key EAEC lineages, we applied multilocus sequence typing to 126 E. coli isolates from a Nigerian case-control study that showed aggregative adherence in the HEp-2 adherence assay, and 24 other EAEC strains from diverse locations. EAEC largely belonged to the A, B1 and D phylogenetic groups and only 7 (4.6%) isolates were in the B2 cluster. As many as 96 sequence types (STs) were identified but 60 (40%) of the EAEC strains belong to or are double locus variants of STs 10, 31, and 394. The remainder did not belong to predominant complexes. The most common ST complex, with predicted ancestor ST10, included 32 (21.3%) of the isolates. Significant age-related distribution suggests that weaned children in Nigeria are at risk for diarrhea from of ST10-complex EAEC. Phylogenetic group D EAEC strains, predominantly from ST31- and ST394 complexes, represented 38 (25.3%) of all isolates, include genome-sequenced strain 042, and possessed conserved chromosomal loci. CONCLUSIONS/SIGNIFICANCE: We have developed a molecular phylogenetic framework, which demonstrates that although grouped by a shared phenotype, the category of 'EAEC' encompasses multiple pathogenic lineages. Principal among isolates from Nigeria were ST10-complex EAEC that were associated with diarrhea in children over one year and ECOR D strains that share horizontally acquired loci.
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