ArticlePloS one2010
Notch and MAML-1 complexation do not detectably alter the DNA binding specificity of the transcription factor CSL.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Bivalent interaction through an intrinsically disordered linker promotes transcription activation complex assembly in Notch signaling.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Changes in searching behaviour of CSL transcription complexes in Notch active conditions.Life science alliance · 2024Article
- The correlation between cancer stem cells and epithelial-mesenchymal transition: molecular mechanisms and significance in cancer theragnosis.Frontiers in immunology · 2024Review
- Enhancers with cooperative Notch binding sites are more resistant to regulation by the Hairless co-repressor.PLoS genetics · 2021Article
- Biophysics of Notch Signaling.Annual review of biophysics · 2021Review
- Delta-like ligand 4-mediated Notch signaling controls proliferation of second heart field progenitor cells by regulating Fgf8 expression.Development (Cambridge, England) · 2020Article
- Targeting Nuclear NOTCH2 by Gliotoxin Recovers a Tumor-Suppressor NOTCH3 Activity in CLL.Cells · 2020Article
- Notch family members follow stringent requirements for intracellular domain dimerization at sequence-paired sites.PloS one · 2020Article
- Activation of the Notch Signaling Pathway In Vivo Elicits Changes in CSL Nuclear Dynamics.Developmental cell · 2018Article
- The Canonical Notch Signaling Pathway: Structural and Biochemical Insights into Shape, Sugar, and Force.Developmental cell · 2017Review
- Genome-wide identification and characterization of Notch transcription complex-binding sequence-paired sites in leukemia cells.Science signaling · 2017Article
- CSL protein regulates transcription of genes required to prevent catastrophic mitosis in fission yeast.Cell cycle (Georgetown, Tex.) · 2016Article
- Notch signaling in cerebrovascular diseases (Review).Molecular medicine reports · 2016Review
- Fission Yeast CSL Transcription Factors: Mapping Their Target Genes and Biological Roles.PloS one · 2015Article
- UniPROBE, update 2015: new tools and content for the online database of protein-binding microarray data on protein-DNA interactions.Nucleic acids research · 2015Article
- Alagille, Notch, and robustness: why duplicating systems does not ensure redundancy.Pediatric nephrology (Berlin, Germany) · 2014Review
- A combination of computational and experimental approaches identifies DNA sequence constraints associated with target site binding specificity of the transcription factor CSL.Nucleic acids research · 2014Article
- Notch transcriptional control of vascular smooth muscle regulatory gene expression and function.The Journal of biological chemistry · 2013Article
- Mastermind-like transcriptional co-activator-mediated Notch signaling is indispensable for maintaining conjunctival epithelial identity.Development (Cambridge, England) · 2013Article
- Epstein-Barr virus exploits intrinsic B-lymphocyte transcription programs to achieve immortal cell growth.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCanonical Notch signaling is initiated when ligand binding induces proteolytic release of the intracellular part of Notch (ICN) from the cell membrane. ICN then travels into the nucleus where it drives the assembly of a transcriptional activation complex containing the DNA-binding transcription factor CSL, ICN, and a specialized co-activator of the Mastermind family. A consensus DNA binding site motif for the CSL protein was previously defined using selection-based methods, but whether subsequent association of Notch and Mastermind-like proteins affects the DNA binding preferences of CSL has not previously been examined. PRINCIPAL
findingsHere, we utilized protein-binding microarrays (PBMs) to compare the binding site preferences of isolated CSL with the preferred binding sites of CSL when bound to the CSL-binding domains of all four different human Notch receptors. Measurements were taken both in the absence and in the presence of Mastermind-like-1 (MAML1). Our data show no detectable difference in the DNA binding site preferences of CSL before and after loading of Notch and MAML1 proteins. CONCLUSIONS/SIGNIFICANCE: These findings support the conclusion that accrual of Notch and MAML1 promote transcriptional activation without dramatically altering the preferred sites of DNA binding, and illustrate the potential of PBMs to analyze the binding site preferences of multiprotein-DNA complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.