ArticlePloS one2010
In vivo activation of the intracrine vitamin D pathway in innate immune cells and mammary tissue during a bacterial infection.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 71 citations in OpenAlex.
- Integrating omics and functional data via representation learning to prioritize candidate genes for pleiotropic effect in dairy sheep.PNAS nexus · 2025Article
- 25(OH)vitamin D inflammatory and oxidative stress markers in healthy Holstein cows and cows with peri-partum diseases during the transition period.Scientific reports · 2025Article
- Intramammary 25-hydroxyvitamin DJDS communications · 2023Article
- Enhancing bovine immune, antioxidant and anti-inflammatory responses with vitamins, rumen-protected amino acids, and trace minerals to prevent periparturient mastitis.Frontiers in immunology · 2023Review
- Stage of infection withFrontiers in veterinary science · 2023Article
- Bovine Immunity and Vitamin DMicroorganisms · 2022Review
- Vitamin DFrontiers in cellular and infection microbiology · 2022Article
- Exogenous Vitamin DFrontiers in cellular and infection microbiology · 2021Article
- Genomic measures of inbreeding coefficients and genome-wide scan for runs of homozygosity islands in Iranian river buffalo, Bubalus bubalis.BMC genetics · 2020Article
- 1,25-hydroxyvitamin D3 decreases endoplasmic reticulum stress-induced inflammatory response in mammary epithelial cells.PloS one · 2020Article
- 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle.BMC veterinary research · 2017Article
- Sodium Octanoate Modulates the Innate Immune Response of Bovine Mammary Epithelial Cells through the TLR2/P38/JNK/ERK1/2 Pathway: Implications duringFrontiers in cellular and infection microbiology · 2017Article
- Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component (GC) gene in clinical mastitis and milk production.Genetics, selection, evolution : GSE · 2016Article
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- Article
- Neonatal calf infection with respiratory syncytial virus: drawing parallels to the disease in human infants.Viruses · 2012Review
- Vitamin D in sepsis: from basic science to clinical impact.Critical care (London, England) · 2012Review
- Vitamin D and sepsis: An emerging relationship.Dermato-endocrinology · 2012Article
- Vitamin D signaling in the bovine immune system: a model for understanding human vitamin D requirements.Nutrients · 2012Review
- Vitamin D binding protein and monocyte response to 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D: analysis by mathematical modeling.PloS one · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Numerous in vitro studies have shown that toll-like receptor signaling induces 25-hydroxyvitamin D(3) 1α-hydroxylase (1α-OHase; CYP27B1) expression in macrophages from various species. 1α-OHase is the primary enzyme that converts 25-hydroxyvitamin D(3) to 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)). Subsequently, synthesis of 1,25(OH)(2)D(3) by 1α-OHase in macrophages has been shown to modulate innate immune responses of macrophages. Despite the numerous in vitro studies that have shown 1α-OHase expression is induced in macrophages, however, evidence that 1α-OHase expression is induced by pathogens in vivo is limited. The objective of this study was to evaluate 1α-OHase gene expression in macrophages and mammary tissue during an in vivo bacterial infection with Streptococcus uberis. In tissue and secreted cells from the infected mammary glands, 1α-OHase gene expression was significantly increased compared to expression in tissue and cells from the healthy mammary tissue. Separation of the cells by FACS9 revealed that 1α-OHase was predominantly expressed in the CD14(+) cells isolated from the infected mammary tissue. The 24-hydroxylase gene, a gene that is highly upregulated by 1,25(OH)(2)D(3), was significantly more expressed in tissue and cells from the infected mammary tissue than from the healthy uninfected mammary tissue thus indicating significant local 1,25(OH)(2)D(3) production at the infection site. In conclusion, this study provides the first in vivo evidence that 1α-OHase expression is upregulated in macrophages in response to bacterial infection and that 1α-OHase at the site of infection provides 1,25(OH)(2)D(3) for local regulation of vitamin D responsive genes.
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