ArticleGenes & development2010
Break-induced ATR and Ddb1-Cul4(Cdt)² ubiquitin ligase-dependent nucleotide synthesis promotes homologous recombination repair in fission yeast.
Article in Genes & development, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 50 citations in OpenAlex.
- Homologous recombination suppresses transgenerational DNA end resection and chromosomal instability in fission yeast.Nucleic acids research · 2023Article
- Chromatin Ubiquitination Guides DNA Double Strand Break Signaling and Repair.Frontiers in cell and developmental biology · 2022Review
- Protective Effect of Thymidine on DNA Damage Induced by Hydrogen Peroxide in Human Hepatocellular Cancer Cells.ACS omega · 2020Article
- Review
- CRL4Cdt2 ubiquitin ligase regulates Dna2 and Rad16 (XPF) nucleases by targeting Pxd1 for degradation.PLoS genetics · 2020Article
- Genomic Instability in Fungal Plant Pathogens.Genes · 2020Review
- Review
- Limited DNA Repair Gene Repertoire in Ascomycete Yeast Revealed by Comparative Genomics.Genome biology and evolution · 2019Article
- The TOR pathway modulates cytoophidium formation inThe Journal of biological chemistry · 2019Article
- An essential role for dNTP homeostasis following CDK-induced replication stress.Journal of cell science · 2019Article
- Cullin Ring Ubiquitin Ligases (CRLs) in Cancer: Responses to Ionizing Radiation (IR) Treatment.Frontiers in physiology · 2019Review
- RRM1 gene expression evaluated in the liquid biopsy (blood cfRNA) as a non-invasive, predictive factor for radiotherapy-induced oral mucositis and potential prognostic biomarker in head and neck cancer patients.Cancer biomarkers : section A of Disease markers · 2018Article
- Set2 Methyltransferase Facilitates DNA Replication and Promotes Genotoxic Stress Responses through MBF-Dependent Transcription.Cell reports · 2017Article
- Article
- Review
- The role of cullin4B in human cancers.Experimental hematology & oncology · 2017Review
- The anti-malarial atovaquone increases radiosensitivity by alleviating tumour hypoxia.Nature communications · 2016Article
- CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection.Nature communications · 2016Article
- The spliceosome-associated protein Nrl1 suppresses homologous recombination-dependent R-loop formation in fission yeast.Nucleic acids research · 2016Article
- Review
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Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
Abstract
Nucleotide synthesis is a universal response to DNA damage, but how this response facilitates DNA repair and cell survival is unclear. Here we establish a role for DNA damage-induced nucleotide synthesis in homologous recombination (HR) repair in fission yeast. Using a genetic screen, we found the Ddb1-Cul4(Cdt)² ubiquitin ligase complex and ribonucleotide reductase (RNR) to be required for HR repair of a DNA double-strand break (DSB). The Ddb1-Cul4(Cdt)² ubiquitin ligase complex is required for degradation of Spd1, an inhibitor of RNR in fission yeast. Accordingly, deleting spd1(+) suppressed the DNA damage sensitivity and the reduced HR efficiency associated with loss of ddb1(+) or cdt2(+). Furthermore, we demonstrate a role for nucleotide synthesis in postsynaptic gap filling of resected ssDNA ends during HR repair. Finally, we define a role for Rad3 (ATR) in nucleotide synthesis and HR through increasing Cdt2 nuclear levels in response to DNA damage. Our findings support a model in which break-induced Rad3 and Ddb1-Cul4(Cdt)² ubiquitin ligase-dependent Spd1 degradation and RNR activation promotes postsynaptic ssDNA gap filling during HR repair.
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