ArticleJournal of clinical microbiology2011
Novel multiplex real-time PCR diagnostic assay for identification and differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis complex strains.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Bovine tuberculosis in Central Ethiopian slaughterhouses and the identification of causative mycobacteria by multiplex real-time PCR.BMC microbiology · 2024Article
- The rapid detection and differentiation of Mycobacterium tuberculosis complex members from cattle and water buffaloes in the delta area of Egypt, using a combination of real-time and conventional PCR.Molecular biology reports · 2019Article
- Cytokine and Chemokine Concentrations as Biomarkers of Feline Mycobacteriosis.Scientific reports · 2018Article
- Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis.The Journal of veterinary medical science · 2017Article
- Differential Identification of Mycobacterial Species Using High-Resolution Melting Analysis.Frontiers in microbiology · 2017Article
- Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood.BMC infectious diseases · 2015Article
- Comparison of Established Diagnostic Methodologies and a Novel Bacterial smpB Real-Time PCR Assay for Specific Detection of Haemophilus influenzae Isolates Associated with Respiratory Tract Infections.Journal of clinical microbiology · 2015Article
- Rapid detection and identification of infectious pathogens based on high-throughput sequencing.Chinese medical journal · 2015Article
- Protein-coding housekeeping gene Rv2461c can be used as an amplification target in loop-mediated isothermal amplification assay for the detection of Mycobacterium tuberculosis in sputum samples.International journal of clinical and experimental pathology · 2014Article
- Importance of the genetic diversity within the Mycobacterium tuberculosis complex for the development of novel antibiotics and diagnostic tests of drug resistance.Antimicrobial agents and chemotherapy · 2012Review
- SeekTB, a two-stage multiplex real-time-PCR-based method for differentiation of the Mycobacterium tuberculosis complex.Journal of clinical microbiology · 2012Article
- Routine use of microbial whole genome sequencing in diagnostic and public health microbiology.PLoS pathogens · 2012Review
- Pyrosequencing assay for rapid identification of Mycobacterium tuberculosis complex species.BMC research notes · 2011Article
- A novel multiplex real-time PCR for the identification of mycobacteria associated with zoonotic tuberculosis.PloS one · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tuberculosis (TB) in humans is caused by members of the Mycobacterium tuberculosis complex (MTC). Rapid detection of the MTC is necessary for the timely initiation of antibiotic treatment, while differentiation between members of the complex may be important to guide the appropriate antibiotic treatment and provide epidemiological information. In this study, a multiplex real-time PCR diagnostics assay using novel molecular targets was designed to identify the MTC while simultaneously differentiating between M. tuberculosis and M. canettii. The lepA gene was targeted for the detection of members of the MTC, the wbbl1 gene was used for the differentiation of M. tuberculosis and M. canettii from the remainder of the complex, and a unique region of the M. canettii genome, a possible novel region of difference (RD), was targeted for the specific identification of M. canettii. The multiplex real-time PCR assay was tested using 125 bacterial strains (64 MTC isolates, 44 nontuberculosis mycobacteria [NTM], and 17 other bacteria). The assay was determined to be 100% specific for the mycobacteria tested. Limits of detection of 2.2, 2.17, and 0.73 cell equivalents were determined for M. tuberculosis/M. canettii, the MTC, and M. canettii, respectively, using probit regression analysis. Further validation of this diagnostics assay, using clinical samples, should demonstrate its potential for the rapid, accurate, and sensitive diagnosis of TB caused by M. tuberculosis, M. canettii, and the other members of the MTC.
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